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@#[摘 要] 目的：检测lncRNA LOC440173在NSCLC组织和细胞中的表达及探讨其对癌细胞恶性生物学行为的影响。方法：选取河北医科大学第四医院生物标本库中2014至2017年手术切除的72例NSCLC患者的癌及癌旁组织标本，应用qPCR法检测NSCLC组织和癌旁组织中，以及6种NSCLC细胞株（H520、H358、A549、HCC827、H1703和H1299）中LOC440173的表达水平；构建LOC440173的敲低及过表达载体，分别转染H520和H1703细胞，应用MTS、克隆形成及Transwell小室迁移和侵袭实验分别检测敲低及过表达LOC440173对NSCLC细胞增殖、迁移及侵袭能力的影响，qPCR法检测LOC440173对于EMT过程相关标志物（E-cadherin、N-cadherin及vimentin）mRNA表达水平的影响，WB法检测其对E-cadherin、N-cadherin蛋白表达的影响。结果：LOC440173在NSCLC组织中的表达明显高于癌旁组织（P<0.01），并与淋巴结转移、组织学分化程度、TNM分期和肿瘤大小有关联（P<0.05或P<0.01）。敲低LOC440173可以抑制H520细胞的体外增殖、迁移和侵袭（P<0.05或P<0.01），过表达LOC440173可显著促进H1703细胞的增殖、迁移和侵袭（P<0.05或P<0.01）。在转录水平上，敲低LOC440173后，E-cadherin的表达水平升高，间充质相关标志物N-cadherin、vimentin的表达水平降低（P<0.05或P<0.01）；而过表达LOC440173后，E-cadherin的表达水平降低，间充质相关标志物N-cadherin、vimentin的表达水平升高（P<0.05或P<0.01）。在转录后水平上，LOC440173负向调节E-cadherin蛋白的表达、正向调节N-cadherin的蛋白表达（均P<0.05）。结论：LOC440173在NSCLC组织中的异常高表达可能与NSCLC的发生发展有关，LOC440173可显著提高NCSCL细胞的体外增殖、迁移、侵袭能力，且其作用机制可能与调控EMT相关基因表达有关。
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Objective@#The study is to explore daily violence exposure and its association with campus bullying, to provide theoretical basis for positive development of middle school students.@*Methods@#Questionnaire survey was conducted by using Violence Exposure Scale, Normative Beliefs about Aggression Scale, Middle School Students’ Self-control Scale, and Middle School Students’ Campus Bullying Scale. During Aug. to Oct. 2019, 1 372 middle school students were selected by the convenient sampling method as subjects of study from 2 junior high schools and 3senior high schools in Xinxiang.@*Results@#The total score in daily violence exposure was (34.22±12.09). The scores of violence exposure, traditional bullying and cyberbullying in female were lower than in male(t=-2.60--6.32, P<0.05). The scores of violence exposure, traditional bullying and cyberbullying in junior high school students were higher than senior high school students(t=4.59-7.50, P<0.05). The relationship between violence exposure and normative beliefs about aggression, traditional bullying, cyberbullying were positive (r=0.20, 0.44, 0.51, P<0.01). The relationship between violence exposure and self-control was negative (r=-0.29, P<0.01) . The relationship between normative beliefs about aggression and traditional bullying, cyberbullying were positive (r=0.28, 0.22, P<0.01). The relationship between normative beliefs about aggression and self-control was negative (r=-0.38, P<0.01). Violence exposure indirectly affects traditional bullying/cyberbullying through normative beliefs about aggression. The effect of normative beliefs about aggression on the traditional bullying/cyberbullying of middle school students is reduced with the increase of self-control.@*Conclusion@#Normative beliefs about aggression plays an intermediary role in violence exposure and traditional bully/cyberbullying, and self-control regulates the relationship between them.
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Objective@#To explore mediating effect of shame on parent-child attachment and bullying among high school students, and to provide the basis for school bullying prevention among high school students.@*Methods@#From September to November 2018, a total of 1 149 students’ bullying behavior from 4 high schools in Shenyang and Xinxiang, were investigated with the Inventory of Parents and Peer Attachment, Middle School Students’ Bullying Scale and Middle School Students’ Shame Scale.@*Results@#About 31.07% of the subjects involved in school bullying. The report rates of bullying among boys, high school students of second grade, only child, students from rural area were higher (P<0.01). Mother-child attachment was negatively correlated with traditional and cyber bullying(r=-0.14, -0.21, -0.20, P<0.01). Father-child attachment was negatively correlated with traditional and cyber bullying(r=-0.19, -0.21, -0.19, P<0.01). Shame was positively correlated with traditional bullying and cyber bullying (r=0.20, 0.19, P<0.01). Shame mediates mother-child attachment, traditional and cyber bullying, accounting for 11.78% and 15.70% of the total effects. Shame mediates father-child attachment, traditional and cyber bullying, accounting for 15.17% and 17.43% of the total effect.@*Conclusion@#Mother-child and father-child attachment have direct effect on school bullying, and indirect effect on school bullying through shame.
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Objective To study the effect of physical activities and dietary intervention on metabolic syndrome (MS) in primary school students.Methods Either one-year physical activities (PA) or dietary intervention was conducted in Grade 1-5 children from eight primary schools in Haidian district,Beijing.A ‘happy 10 minutes' program was held in the PA group,while the dietary group receiving nutrition lectures.Baseline and post-intervention data on height,weight,waist circumference,serum lipids,glucose,and blood pressure were collected.Results The prevalence of MS at baseline was 9.0％.After intervention programs were carried out,improvements on triglyceride,high density lipoprotein,fasting blood glucose,diastolic blood pressure and waist circumference in the dietary group or PA group were seen,with the dietary group showed better effects than the PA group.The prevalence of MS decreased from 10.4％ to 4.6％ in the dietary group with statistically significant difference,while it increased in both the PA group and the control group.Girls showed better effects in the dietary group.Older students showed better effects than the younger students in the PA group.In non-overweight and overweight students of the dietary intervention group,the MS related components were significantly improved.Conclusion The prevalence of metabolic syndrome was relatively high in the primary school students in Beijing.Education on nutrition could reduce the prevalence and improve the related components,which seemed to be more effective than in the PA intervention.Sex,age and nutritional status were the confounding factors for intervention programs.