ABSTRACT
Controlling risk factors such as dyslipidemia in patients with coronary artery disease, including candidates for coronary artery bypass grafting [CABG], is of great importance and has serious effects on CABG morbidity and mortality. The aim of this study was to evaluate lipid serum levels, comprising TG, LDL, and HDL, before CABG and their relation with in-hospital outcome. The clinical profiles of 3,593 patients in the hospital cardiac surgery databank who underwent isolated CABG between April 2006 and April 2008 were reviewed. Three components of lipid profile, including TG, LDL, and HDL serum levels, were checked all the time of hospitalization in all the patients. Lipid control was evaluated according to the published guidelines. The mean LDL, HDL, and TG serum levels were 103.4 +/- 48.5, 40.9 +/- 16, and 168 +/- 87 mg/dl, respectively. Additionally, 487 [13.6%] patients had entire TG, LDL, and HDL serum levels within the acceptable range and in 668 [18.6%] patients, all of these components were within the uncontrolled range. After adjustment for confounders, in-hospital mortality in patients with uncontrolled TG, LDL, and HDl was higher than patients with controlled TG, LDL, and HDl [p value=0.042, OR=1.299, 95% CI =1.012-1.934]. The high prevalence of uncontrolled lipids in our patient population is alarming. Regular and frequent pre- and post-operative visits to monitor and modify patient risk factors, including dyslipidemia, seem necessary. An increase in stain dosage or adjunctive therapy with other lipid lowering agents may be helpful. Attempts to maintain all lipids the controlled range may have beneficial effects on hospital outcome
ABSTRACT
Background: Purity of cultured Schwann cell is very important in patients' outcome. The purpose of this study was evaluation of cell purity in a culture without nerve growth factors and fetal bovine serum
Materials and Methods: In this experimental study, for culture of human Schwann cells, nerve growth factors and fetal bovine serum were replaced by human autologous serum. Obtaining a consent from the close relative, nerve grafts were harvested and transported to processing unit where they were cultured in DMEM upon aseptic condition. Then the cultured cells were evaluated with S100 antibody staining for both morphology and purity
Results: Cell purity range was from 97% to 99% [mean=98.11+/-0.782%]. Cell count was 14055.56+/-2480.479 per microliter. There was not significant correlation between cell purity with either the culture period or the age of donors [P>0.05]. The Spearman correlation coefficient for the cell purity with the culture period and the age of donors was -0.21 and -0.09, respectively
Conclusion: We found that the replacement of nerve growth factors and fetal bovine serum with human autologous serum improves the cultured Schwann cells for clinical use with more safety and minimum reagents