ABSTRACT
To examine the in vitro antibacterial activity of the ethanol extract of fresh fruits of Solanum incanum L., fresh leaves of Ricinus communis L. and Allium ampeloprasum var. porrum L., and to determine and quantify the phenol compounds of the investigated plant parts. This study was carried out at the Faculty of Science, King Khalid University, Abha, Kingdom of Saudi Arabia from July 2011 to September 2011. Nine clinical strains of bacteria and 3 ethanol extracts of 3 plant species were used for the antimicrobial study. Thirty grams of each sample was ground, filtrated, and each filtrate mixed with 100 ml ethanol and placed in a shaker for 48 hours. The ethanol was evaporated from the sample, weighed, and subjected to an antibacterial activity test using the agar diffusion method. The high-performance liquid chromatography was used to identify and quantify the phenols extracts of investigated samples. Ethanol extract of the investigated plant parts showed antibacterial activities against different pathogenic bacteria. Leaf extracts of Ricinus communis showed the highest antibacterial activity, followed by the leaves of Allium ampeloprasum var. porrum, while the fruits of Solanum incanum showed the least activity. The amounts of main phenols detected in Ricinus communis leaves were higher than those of Solanum incanum fruits and Allium ampeloprasum var. porrum leaves. The ethanol extract of the tested plants could be considered as an alternative source of new antibacterial drugs
ABSTRACT
To investigate the antibacterial effect of the crude latex of Argemone ochroleuca [A. ochroleuca] as antibacterial potential against a range of human pathogenic bacteria. This study was carried out at King Khalid University, Abha, Kingdom of Saudi Arabia from January to March 2010. Seventeen ml of fresh latex from A. ochroleuca Sweet was collected, and the antibacterial activity of crude and diluted latex were examined using one ml of standardized inoculum suspension, and using the agar diffusion method test against Bacillus subtilis, Enterobacter aerogenes, Micrococcus luteus, Escherichia coli, and Staphylococcus aureus. All inoculated plates were incubated aerobically at 29 [degree sign] C for 48 hours. The diameter of the zones of inhibition was measured to the nearest mm. the crude latex of A. ochroleuca exhibited a potent antibacter effect on all bacterial strains examined. The zones of inhibition against the tested bacteria were found in the range of 9.30 - 40.3 mm along with their respective minimum inhibitory concentration values 100 micro l/ml. The observable inhibition on selected bacteria by latex of A. ochroleuca makes it a promising alternatives as a potential source of natural antibacterial