ABSTRACT
Objective: To determine the effects of George Ohsawa One [GO1] on sodium, potassium, calcium, hemoglobin and platelet count in rabbits with and without anticoagulated blood
Study Design: Interventional study
Place and Duration of Study: This study was conducted at the Department of Pharmacology, FederalPost Graduate Medical Institute and National Health Research Complex, PMRC, Sheikh Zayed Hospital Complex, Lahore from January 2010 to August2011
Materials and Methods: Forty eight [n=48] male rabbits were included in the study. They were divided into two groups which were further divided into two subgroups each. About 700 grams of GO1 was used in the study
Results: The effect of GO1 on platelet count, hemoglobin, serum sodium, potassium and calcium were found to be insignificant
Conclusion: This work on the effect of GOI is first of its kind on biochemical and hematological parameters other than prothrombin time and activated partial thromboplastin time in rabbits measured quantitatively. Although theuse of herbal products may not be dangerous per se, further research work is required to explore the benefits of GO1 on biochemical and hematological parameters before it can safely be used in humans as an anti-hemorrhagic agent
ABSTRACT
Alloxan monohydrate is a common drug used for developing experimental diabetes in animals, as streptozotocin, though less toxic than alloxan is considerably costlier. This study aimed to provide an exact and detailed account of alloxan induced diabetes in rabbits as none was available in both local and international literature and in Pharmacology textbooks. This resulted in extremely high mortality in experimental animals i.e., rabbits, leading to inevitably wasted research time and effort for a researcher.Twenty-four healthy rabbits of a local strain weighing 1000 to 1800 g were obtained and kept at the animal house of the Department of Pharmacology Shaikh Zayed Hospital, Lahore. They were injected with varying doses of Alloxan monohydrate [from 75mg -1 l0mg]/ kg body weight in order to induce diabetes. The blood glucose RBS levels were estimated 8 days after injecting the alloxan and subsequently randomly at intervals of five to ten days till the rabbits became diabetic. These doses were found to lower the rabbit mortality by 25% as compared to [upto 75%] that was encountered with a single standardized dose of 160mg/kg body weight suggested by Akhtar et al. [1982] or 80mg/kg body weight [Puri and Prabhu 2002]. It was therefore concluded that our proposed regime may be beneficial for future researchers aiming to develop a similar animal model
ABSTRACT
To find out the frequency of hepatitis B, C and human immunodeficiency virus [HIV] in blood donors at Shaikh Zayed Hospital, Lahore. Design: This is a non-interventional study. Study Period: Period of this study is from 1-1-2005 to 30-6-2005. Study was conducted at the Blood Bank of Shaikh Zayed Hospital, Lahore. Subjects and Methods: In this study 18216 young healthy voluntary blood donors [age 16-50 years] comprising of 16611 males and 1605 females were included and tested for HBsAg, Anti-HCV and HIV by Accu check one step test [chromatographic immunoassay] in serum. Results: Frequency of hepatitis B, C and HIV infection was 3.36%, 4.16% and 0% respectively. We can get representative prevalence values of HBV, HCV and HIV infection in general population by collecting data from all blood transfusion centers of Pakistan. This is also helpful to create public awareness regarding hepatitis and HIV transmission through blood transfusion