ABSTRACT
Introduction:-Systemic lupus erythematosus is an autoimmune disease characterized by inflammation of many different systems. Epidemiological studies showed an increase of cardio and cerebrovascular events in patients suffering from systemic autoimmune diseases, and autoptic investigations pointed out that an accelerated atherosclerotic process is largely responsible for such manifestations. Both symptomatic as MI [myocardial infarction] and asymptomatic as atherosclerotic [carotid plaque] diseases are more prevalent in SLE patients than in the general population. There were positive correlations between CD69 [one of type I IFN-regulated genes in platelets] level and cardio vascular complications in these patients
Objective:-To determine the level of CD69 andC3 in patients with systemic lupus erthematosus and to find the relationship between CD69 andC3 levels and cardio vascular complications in these patients
Methods:-Twenty systemic lupus erthromatosis patients were included in this study were selected from internal medicine in and out-patient clinics of Al-Zahraa university hospital, ten patients suffering of SLE with no cardio vascular disease [Group1] and ten patients suffering of SLE with cardio vascular disease [Group 2], Ten healthy individuals of matched age and sex were selected as control group. All systemic lupus patients were subjected to full history taking, clinical examination, ECG, complete blood picture, liver function, renal function, ANA, dDNA, serum complement 3, and CD69 on platelets were done
Results:-The expression ofCD69 on platelets was significantly higher in patient of SLE with cardiovascular complication group than control group[p0.0071],and there was a significant increases in the serum level of C3 of SLE with cardiovascular complication group than in patient of SLE without cardiovascular complication group [p=0.000005]. Also we found that the serum level of C3 lower in patient of SLA group with and without cardiovascular complication than control group [p=0.009] [0.00009]
Conclusion:-These finding suggest that Platelets with CD69 expression seem to more activated and may contribute to development of vascular complication in patients of SLE so CD69 expression on platelets may serve as predictive marker of CVD complication in patients with SLE
ABSTRACT
To evaluate the role of procalcitonin [PCT], high senstive C-reactive protein [hs-CRP] and Interleukin-6 [IL-6] for prediction of subclinical intrauterine infection in pregnant women with preterm premature rupture of membranes. Twenty patients with preterm premature rupture of membranes PPROM [study group] and twenty apparently healthy pregnant women [control] group between 26-34 weeks of pregnancy were enrolled in this study. In all cases analysis of serum procalcitonin by high performance liquid chromatography, Il-6 by ELISA method and hs-CRP by nephelometry were done. Culture of vaginal bacteria was done for study group only. Procalcitonin levels in the PRROM group were significantly higher than in healthy pregnant women [median 1.95 versus 0.39-Interquartile range 1.375 versus 0.213 - P0.001]. A significant correlation was observed between PCT and hs-CRP [r=0.510; P0.031] and leucocytosis [r=0.544- P 0.013]. Also IL-6 levels were significantly higher in cases of PPROM compared with control group [median 40.01 versus 5.55 - Interquartile range, 45.88 versus 4.22 P 0.001]. No significant correlation was present between PCT and IL-6. Determination of PCT, hs-CRP and IL-6 in mother's blood sample can be useful for diagnostics of PPROM cases suspected of intrauterine infection. However PCT more valuable and specific
Subject(s)
Humans , Female , Adolescent , Adult , Uterine Diseases , Pregnant Women , Calcitonin/blood , C-Reactive Protein , Interleukin-6/blood , Enzyme-Linked Immunosorbent Assay , ChromatographyABSTRACT
Background: a potential role of toxoplasmosis for immune dysfunction has been suggested in Autism spectrum disorder [ASD]. The imbalances in pro- and anti-inflammatory processes could markedly hinder. host defense mechanisms important for immune control of the Toxoplasmosis. To test this hypothesis, we investigated evidence of differential cytokines release in plasma samples obtained from 3 to 10 year-old children with ASD compared with age-matched typically developing [TD] children with toxoplasmosis and without toxoplasmosis
Methodology: twenty four [18 boys and 6 girls] autistic children were included in this study. Their age ranged from 3- 10 years old [mean = 69.25 +/- 25.6 month]. There were diagnosed according to DSM 1V criteria. Control group included eighteen non-autistic children. The control group was divided in to two groups 1] control with toxoplasmosis [9 children]. 2] Control without toxoplasmosis [9 children]. All were subjected to uull history, clinical examination, and estimation of serum Toxoplasma IgG, serum TNF- alpha, INF -gamma and catalaze enzyme
Results: sex autistic children were Toxoplasma IgG positive [25%]]. Level of catalase enzyme was significantly lower in all patient than the control [P =0.048] with no significant differences of TNF- alpha and INF-gamma [P = 0.272, = 0.137] respectively. Serum TNF- alpha -level in autistic children with toxoplasmosis was highly significantly correlated with severity of autistic criteria assessed by Childhood Autistic Rating Scale [CARS] [r = 0.986, P=.0.000]. There was no significant correlation of INF-gamma and catalase level with severity of autistic criteria [r= 0.312, P= 0.5] [r= 0.720, P= 0.189]. INF-gamma and catalase levels were significantly correlated with severity of autism [r= - 0.496, P= 0.036] and [r= 0.650, P= 0.004] its autistic children without toxoplasmosis. TNF level was not significantly correlated with total CARS [r =000, P=l] in the same group
Conclusion: toxoplasmosis may result in immune modulation among ASD patients. These fluctuations in cytokines could result in a recurrent profuse multiplication of Toxoplasma gonadi in the brain associated with persistent neuroinflammation