Cytogenetic alterations in human lymphocyte culture following exposure to radiofrequency field of mobile phone.

The present study aims to address the possible cytogenetic effect of mobile phone on human lymphocyte culture. Human peripheral blood cultured from healthy, non-smoking donors exposed to 1950 MHz and safety limit (2w/kg) of absorption rate (SAR) mobile radiofrequency radiation for 5, 10, 15, 20, 25 and 30 min, then harvested after 24 hr after subjection. The alkaline comet assay, chromosomal aberrations and the micronucleus test were used, to check for changes, stress response and alterations in lymphocytes. The result indicated the presence of timedependant cellular response to RF exposure of mobile phone kept in the standby position, through comet tail factor, DNA fragmentation, chromosomal aberrations and centromeric negative nuclei (MN) in human lymphocyte culture. This effect may be attributed to oxidative stress induced by mobile phone radiation.

use of single cell gel electrophoresis [comet] assay to detect genetic damage in clarias gariepinus erythrocytes treated with malathion

Pollution of environmental waters is a serious and growing problem all over the world. Fish accumulate pollutants directly from contaminated water and indirectly by ingestion of the contaminated organisms. Genotoxic pollutants induce DNA strand breaks and mutagenic lesions that proposed as genotoxicity biomarkers monitoring the fresh water environment. These lesions are believed to induce chemical carcinogenesis. In the present communication,the alkaline single cell gel electroplioresis,known as cornet assay, is a rapid,simple and sensitive technique measuring DNA strand breaks in individual cells. The genotoxic potential of the widely used pesticide [Malathion] was evaluated in clarias gariepinus erythrocytes. The cells were exposed to three sub-lethal concentrations of malathion [0.02, 0.3 and 0.5 micro g/ml]. Alkaline comet assay was performed on nucleated erythrocytes after 24,48 and 72hrs. The amount of DNA damage in cells was estimated from comet tail length as the extent of migration of the DNA damaged. DNA damage was observed to be increased with the increase of the concentration compared to the control [p<0.05]. The comet tail showed a concentration - time relationship. The maximum tail length at highest concentration and longest time was [8.62 micro m]. The present study indicated that comet assay applied on fish erythrocytes is a useful tool in estimating genotoxicity of chemical pollutants, such as malathion.

Correlation between lifestyle profile and health related indicators among middle age working women

This study was carried out to describe the lifestyle profile behavior of middle age working women, and examining the association between lifestyle behavior and the presence of health related chronic conditions. This study was carried on 125 non pregnant middle age [30-60 years] working women from 4 different work sites. Among the important studied issues of lifestyle practice assessed using a structured interview questionnaire that modified and revised by the researchers nutritional practice, physical exercise, rest and sleep practice, stress management, health care practice, and health perception. The tool also include other items about demographic characteristics, present health status and health status indicators [Body Mass Index [BMI], blood pressure, and blood analysis for blood glucose, cholesterol and hemoglobin that assessed by the researchers]. The data from the interviews were analyzed using SPSS, yielded frequencies, mean, standard deviation, pearson's product moment correlation and one way analysis of variance [ANOVA]. The findings of this study revealed that the mean age of women was 41.01 +/- 6. The practice of physical exercise was the lowest lower score, only .8% practicing regular exercise, and 68.7% have moderate perception of their health, even the healthy women. Also, a positive correlation was found between higher nutritional score and practicing exercise. About 36.8% have chronic condition, 16% of conditions are detected and referred. WHO categorization of BMI [normal < 25, overweight > or = 25 < 30, obese > or = 30 < 39, morbidly obese > or = 39] was used to assess obesity, 75.2% of participants were found to be obese or morbidly obese, the mean BMI was 33.29 +/- 5.08. There was a positive correlation between BMI and elevated systolic and diastolic blood pressure. Also a positive correlation between elevated systolic and diastolic blood pressure and high cholesterol level. In conclusion, lifestyle practice continues to have a significant impact on women's health status. Nursing interventions are essential to enhance health lifestyle practice among middle age working women