ABSTRACT
Objectives—We tested the effect of a clinically applicable dose of moxibustion on adjuvant-induced arthritis (AIA) of rat, an experimental model of rheumatoid arthritis.<br>Methods—Male Lewis rats were inoculated with <i>Mycobacterium butyricum</i> suspended in paraffin oil into the right hind paw to induce arthritis. Moxibustion (60°C) was applied to the right hind limb point, Tsu-san-Li (ST36), twice a week for 4 consecutive weeks. The efficacy of the above treatment was determined by the measurements of paw swelling, arthritic score. The effects of moxibustion upon immune and inflammatory responses were analyzed by enumerating peripheral blood leukocyte subsets. The data were analyzed with Mann-Whitney U-test between the experimental and control groups.<br>Results—Moxibustion significantly suppressed paw swelling in the systemic phase, but not in the acute phase, of arthritis. Moxibustion also significantly suppressed the increase in T lymphocyte numbers in the late acute phase and that of neutroplils in the systemic phase.<br>Conclusion—After the treatment with moxibustion, significant alterations were observed in the numbers of peripheral blood leukocyte subsets in AIA, along with the amelioration of clinical signs. These observations suggest that suppression of AIA with moxibustion may be mediated through the suppression of proliferating number of T-cell and acceleration of decrease in number of neutrophils in the peripheral blood.