ABSTRACT
Objectives: To investigate hepato-protective effect of ethanolic leave extract of Raphanus sativus against atorvastatin induced hepatotoxicity in albino rabbits
Study design: Experimental study
Material and Method: This study conducted at department of Physiology and Pharmacology, University of Agriculture, Faisalabad. The ethanolic extract of Raphanus sativus was prepared for hepatoprotective effect against hepatotoxicity produced by atorvastatin
Results: The results were compared with normal and experimental control. Serum was tested for ALT, AST, Alkaline Phosphatase and Bilirubin along with histological studies. Ethanolic extract of Raphanus sativus with 100mg/kg. of body weight dosage developed some changes representing hepatotoxicity but higher doses showed an increase in toxicity as observed on histologic sections. A reduction in fibrosis and cholestasis was observed which needs further evaluation
Conclusion: Ethanolic extract of raphanus sativus did not show any amelioration in the increased enzymes level however a reduction in cholestasis was observed as represented by decreases in alkaline phosphatase levels also confirmed by histological studies
ABSTRACT
There are no effective methods to treat thrombocytopenia once it occurs. Transfusions, growth factor injections and bone maiTow transplant have their limitations. So there is increased need for research of drugs that could prevent and treat thrombocytopenia. The objective the study to deter-mine the effect of different doses of male and female papaya leaf juice on prevention of carboplatin induced thrombocytopenia in mice. A total of 55 Swiss albino mice were randomly divided into five groups [C, M[10], M[5], F[10] and /y. Thrombocytopaenia was induced in all groups by a single intraperitoneal injection of carboplatin. Male papaya leaf juice was given to prevent of thrombocy topaenia to groups M[w] and M[5] and female papaya leaf juice was given to F[w] and F[5]. On days o, 7,14 and 21 blood samples were collected by cardiac puncture for platelet count. Significance of difference was calculated by one wayANOVA. After carboplatin injection, platelet count decreased. Papaya leaf juice prevented fall in platelet count throughout the study period with p-value < 0.001. Difference between male and female papaya leaf juice was not significant while higher dose [10 ml/kg] produced significantly higher responses as compared to low dose [5 ml/kg]. Papaya leaf juice prevents reversible thrombocytopaenia induced by carboplatin in a dose dependent manner. There is no difference between male and female plants in this respect
ABSTRACT
Tobramycin, an aminoglycoside, and piperacillin, an antipseudomonal penicillin, are widely used to treat Gram -ve infections. Their concurrent use is recommended because of their synergistic action and prevention of resistant strains against monotherapy. This experimental work was aimed to study the effects and interaction of these important drugs on rabbit kidney. Male rabbits were administered tobramycin, piperacillin, and tobramycin plus piperacillin for 21 days [n = 6 in each group]. Blood and urine samples were collected on day O, 11, and 21. Blood was analyzed for BUN, serum creatinine, serum potassium, and serum sodium while urine was analyzed for urine volume, creatinine, albumin and specific gravity. Renal creatinine clearance was calculated. Results showed some change in renal function with use of tobramycin but piperacillin neither changed the renal function nor did augment the toxic effect of tobramycin so it was concluded that combination of both drugs in safe