ABSTRACT
Aim: The aim of this study was to design multi-walled carbon nanotubes [MWCNTs] loaded with paclitaxel [PTX] anti-cancer drug and investigate its anti-cancerous efficacy of human gastric cancer
Background: Carbon nanotubes [CNTs] represent a novel nano-materials applied in various fields such as drug delivery due to their unique chemical properties and high drug loading
Patients and Methods: In this study, multi-walled carbon nanotubes [MWCNTs] pre-functionalized covalently with a paclitaxel [PTX] as an anti-cancer drug and evaluated by different analyses including, scanning electron microscope [SEM], particle size analyzer and cellular analyses
Results: A well conjugated of anti-cancer drug on the carbon nanotube surfaces was shown. This study demonstrates that the MWCN-PTX complex is a potentially useful system for delivery of anti-cancer drugs. The flow cytometry, CFU and MTT assay results have disclosed that MWCNT/PTXs might promote apoptosis in MKN-45 gastric adenocarcinoma cell line
Conclusion: According to results, our simple method can be designed a candidate material for chemotherapy. It has presented a few bio-related applications including, their successful use as a nano-carriers for drug transport
ABSTRACT
Poly vinyl alcohol/Chitosan nanofibrous mat were prepared by electrospinning method with suitable pore sizes as potential matrices for soft tissue engineering. The designed scaffolds by electrospinning method evaluated by differentanalyses such morphological, mechanical, and cellular analysis.Microscopic results showed diameters of poly vinyl alcohol/Chitosan nanofibers were approximately 150 nm. Mechanical investigations illustratedstress - strain curve of poly vinyl alcohol /chitosan mat indicate good flexibility with average strain and good percentage of yield stress. The cellular resultsrevealthat addition of chitosan to poly vinyl alcohol enhances viability and proliferationof fibroblast cells, which increases the biocompatibility of the scaffold. In fact, addition of a smallpercentage of chitosan to the poly vinyl alcoholproved to be a promising approach for designof a scaffold
ABSTRACT
Background: There are various factors that may cause pain and dysfunction in the pelvic region. Myofascial trigger points can likewise contribute in pelvic pain. Common treatments for myofascial trigger points include electrotherapy, laser therapy, massage, ischemic compression, dry-needling, stretch, icing, heating, and biofeedback
Case Report: A 26 year old man with an exertion-related pain that lasted 5 months was referred for physiotherapy consultation. He had no pain at rest but reported a referral pain from perineal region to the anus and muscular stiffness following a bout of physical activity. On palpation there was a trigger point in the perineal region with referral pain to anus. At the beginning of the treatment, the patient was asked to stop his physical activities. The patient received a treatment package which was useful in the management of trigger points. After 7 sessions of treatment the pain was diminished and there was no exercise induced stiffness. The patient was followed for 10 months later and no pain and stiffness was reported
Conclusion: The application of heat, friction massage, stretching, combined with endurance exercise could be an effective treatment for reliving the pain and muscular stiffness caused by trigger points
ABSTRACT
This article reviews the recently published data on the diagnosis of cancer with proteomics, including the major proteomics technologies and promising strategies for biomarker discovery and development. Most of the tumor markers are proteins that either numerically increase in response to the alteration of cancer conditions or are produced by cancer cells. However, they are natural compounds ordinarily available in the typical cells to a little extent what are affected by increase of expression due to cancer and its intensity in blood, body fluids or tissues. Tumor markers are substances normally available in body fluids such as serum, urine, blood, and tissues that increase in the desired tissue of cancer patients. Most of tumor markers are proteins that either are produced in response to changes in cancer conditions or are made by the cancer cells. However, most of tumor markers are among the natural compounds of normal cells present in normal conditions in the cell in small amounts and are affected by increase of expression, due to cancer and their levels in the blood, body fluids or tissues
ABSTRACT
Evidence is emerging for the association of polymorphisms in PRODH gene and increased risk of schizophrenia. In this project, peripheral blood sampling was obtained from 175 schizophrenia patients that their diseases were confirmed by psychiatrists. 185 healthy individuals were considered as control group. The related tests were administered on the basis of PCR-RFLP. In the continuation, statistical analysis was made on the basis of obtained genotypes from two groups of healthy and patient groups using SPSS16.0 software. The administration of this project aims at investigating the hypothesis that proline dehydrogenase gene, as one of the most important genes involved in schizophrenia incidence which is proved in various populations [outside Iran], may also be involved in incidence of schizophrenia in Iran. This study has analyzed one single nucleotide polymorphism in the PRODH gene, including 757C/T in the incidence of this disease in the given statistical population. According to our results, SNP 757 C/T may be effective in incidence of the disease since P value was < 0.01. Ultimately, our results suggest that outbreak of mutation in PRODH gene in our population can be one of causes of increasing risk of Schizophrenia in this population
ABSTRACT
Schizophrenia is a mental disorder painful with prevalence of about 1% in the world. Nowadays, there is no experimental test aiding the accurate diagnosis of schizophrenic patients but recently quantitative determination of some serum proteins led to many investigations on the roles of these in neuropsychiatric.In the present study, our aim is to quantitative determination ApoA1 and IgM between 134 schizophrenic patients and 127 healthy control persons. Schizophrenia patients were selected from the Tehran Razi hospital. The age [p=0.53] and sex distributions of schizophrenic patients were similar to those of control persons. We measured ApoA1 and IgM levels by immunoturbidimetry method. P<0.05 was considered significant. ApoA1 showed a significant decrease in serum [p=0.00] and IgM to be significantly increased [p=0.00].Furthermore, female patients showed an increase IgM more than males. These results for ApoA1 and IgM support other reports. Decrease ApoaA1 confirmed the relationship of lipid metabolism in schizophrenia patients, also increase IgM evaluate the impact of treatment. This report can help to identify disease markers and treatment such as immunoglobulin therapy or regulate lipid metabolism. It can be imagined that immunoglobulin therapy more effective in the female patients than male and need to expand about them
ABSTRACT
There are several types of cancer, which cause millions of deaths worldwide every year. Many studies have confirmed that plants are adequate natural sources to be examined as anti-cancer drugs with fewer side effects than chemotherapy and radiotherapy. In this study the anti-cancer properties of Lavender aqueous extract on lymphocytes derived from patients with Hodgkin's lymphoma has been studied. In order to determine the cytotoxic effects of the extract on lymphocytes of patients in stages III and IV of Hodgkin's lymphoma and two different cell lines in the presence of different concentrations of aqueous extract of Lavender, MTT colorimetric assay and flow cytometry analysis were used. Findings indicated that Lavender inhibited cell proliferation in both lymphocytes and cell lines with different effects. The effective concentration of Lavender that decreased viability of Hodgkin's lymphoma cells below Lethal Concentration 50 [LC50] value was 100 micro g/ml and this was half of the therapeutic dose. In addition, apoptosis was the main mechanism the Hodgkin's lymphoma cell encountered when exposed to the aqueous extract of Lavender. Conclusion: This experiment proposes that aqueous Lavender extract can be regarded as a potential anti-cancer agent in future studies
Subject(s)
Humans , Plant Extracts , Hodgkin Disease , Lymphocytes , Cell Proliferation , Cell Line , CytotoxinsABSTRACT
Due to increasing clinical demand for adipose tissue, a suitable cell for reconstructive adipose tissue constructs is needed. In this study, we investigated the ability of Human Endometrial-derived stem cells [EnSCs] as a new source of mesenchymal stem cells to differentiate into adipocytes. EnSCs are the abundant and easy available source with no immunological response, for cell replacement therapy. Single-cell suspensions of EnSCs were obtained from endometrial tissues from 10 women experiencing normal menstrual cycles, and were cultured at clonal density [10 cells/cm[2]] or limiting dilution. Endometrial mesenchymal stem cell markers were examined flow cytometry. These cells were treated with adipogenicinducing medium for 28 days. The adipogenic differentiation of the EnSC was assessed by cellular morphology and further confirmed by Oil Red O staining and RTPCR. The BM-MSC differentiated into adipocytes in the presence of adipogenic stimuli for 3 weeks. The flow cytometric analysis showed that the cells were positive for CD90, CD105, CD146 and were negative for CD31, CD34.We showed that the key adipocytes marker PPARa was expressed in mRNA level after 28 days post treatment [PT]. According to our finding, it can be concluded that EnSCs represent a useful in vitro model for human adipogenesis, and provide opportunities to study the stages prior to commitment to the adipocyte lineage
Subject(s)
Humans , Female , Stem Cells , Endometrium , Mesenchymal Stem CellsABSTRACT
A functional treatment for skeletal damages in orthopedic and oral maxillofacial surgeries is required. Platelet growth factors such as Platelet Derived Growth Factor [PDGF], Bone Morphogenic Factor [BMP], Transferring Growth Factor-beta [TGF-beta] and Insulin-like Growth Factor-1 [IGF-1] precede wound healing and bone regeneration. In the present study we focused on the effect of platelet rich plasma [PRP], platelet rich plasma gel [PRP-Gel] and auto bone chips on this process. 30 male, 22 weeks old, Sprague-Dawley rats weighing 525 g were used. They were divided in three groups consisting of PRP [treated by Platelet-Rich Plasma], PRP-Gel [treated by it], Bone chips and Control [two cavities created in each animal in this group]. After 16 weeks they were histologically investigated while in the periods of 40, 60, 90and 120 days, the radiography had been done. The radiographic analysis showed complete treatment in all groups; however, by the histo-pathological investigations by auto bone chips complete and PRP-Gel partial healing has been observed. By histo-morphometric surveys [100+/-25] % in bone chips and [50+/-25] % in PRP-Gel groups bone bridging were observed, whereas in PRP it was not noticeable. The Present study suggests that neither PRP, nor PRP-Gel could be as beneficial as bone chips. Statistically, in PRP-Gel group, due to the existence of fibrin and thrombin, solid bone bridging at the treated site is indicated. According to the previous studies, in which the key role of both inhibitory and stimulatory signals in controlling the bone regeneration were proven, we suggest that auto bone chips could completely enhance healing due to signals among blood factors, environmental tissues and skeletal particles
ABSTRACT
Embryonic stem [ES] cells are derived from the pluripotent inner cell mass [ICN] cells of blastocysts with the potential to maintain an undifferentiated state indefinitely. The derivation process involves plating of the blastocysts on mouse embryonic fibroblast [MEF] and expansion of the outgrowth in to established ES cell line. ES cell are capable of unlimited self-renewal by symmetric division and differentiated cells to all primitive embryonic germ layers. The capacity of ES cells to differentiate in to almost all the cell types of human body highlights their potential to play a promising role in cell replacement therapies for treatment of human diseases. In this study, MEFs have been replaced with human mesenchymal stem cells [hMSCs]. C4 mES cell [mouse embryonic stem cell line] colonies are cultured on inactivated hMSCs amplified >/= 600-folds during the 30 days of continuous culture. The longest continuous expansion of C4 mES cells on hMSC was 30 passages. In this study the gene expression for Oct-4, Nanog, Rex1, Brachyury, LIF, LIFR, TERT, B2M, Stat3, Sox2, Fgf4 in mES cells using reverse transcriptase polymerase chain reaction [RT-PCR] and in which genes expression for Stat3, Sox2, Fgf4 genes was negative whilst the gene expansion for Oct-4, Nanog, Rex1, Brachyury, LIF, LIFR, TERT, B2M genes was positive. There was also a karyotype analysis for ES which showed normal result. The immunocytochemical analysis of Oct4 transcriptional factor for ES cells was made which showed positive result for this factor. These genes may be novel candidates to play critical roles in the regulation of ES Cell pluripotency and self-renewal