new RP-HPLC method for analysis of mebeverine hydrochloride in raw materials and tablets

A simple, sensitive, selective, reliable, least time consuming and rapid high-performance liquid chromatographic method for the determination and quantification of meheverine hydrochloride using hyoscine butylbromide as internal standard has been developed. The chromatographic system consisted of a Shimadzu LC-10 AT VP pump, SPD-10 AV VP UV visible detector, and a CBM-102 Bus Module integrator. Separation was achieved on the micro Bondapak 125 a C18 10 micro l column at room temperature. The samples were introduced through an injector valve with a 10 micro l sample loop. Acetonitrile-water [1:1 v/v] was used as mobile phase, with flow rate 1.7 ml/minutes. pH was adjusted to 2.9 with phosphoric acid. U.V detection was performed at 205 nm. The results obtained showed a good agreement with the declared content. Recovery values of mebeverine hydrochloride were from 99.80% to 100.13%. The proposed method is rapid, accurate, and selective and may be used for the quantitative analysis of mebeverine hydrochloride. The method was found to be specific, accurate, precise and reliable for the determination and quantification of mebeverine hydrochloride in form of raw materials, in bulk drugs and formulation. It was possible to determine all of them in the concentration range of 5-30 nano grams. The detection limit of mebeverine hydrochloride was 0.4-nano gram

In vitro release of tetracyclines in presence of H[2] - receptor antagonists

There are a number of drug interactions reported for tetracyclines with many other drugs. In present study in vitro release of five different tetracyclines i.e., tetracycline, demecloctcycline, oxytetracline, methacycline and doxycycline in presence of cimetidine and ranitidine has been studied by USP XX dissolution method. The release of almost all tetracyclines was depressed in presence of cimetidine and ranitidine while in some cases the value were greatly increased due to the formation of charge transfer complexes of the antibiotic with H2-receptor antagonists, which gave absorbance at same wavelengths but with much higher molar absorptivity. An attempt was made to elucidate the mechanism of this effect

Determination and quantification of cetirizine HCL in dosage formulations by RP-HPLC

A simple, sensitive, reliable and rapid HPLC method for the determination of cetirizine hydrochloride using hyoscine butyl bromide as an internal standard has been developed. The chromatographic system consisted of Shimadzu LC-10 AT VP pump, SPD-10 AV VP with UV/visible detector and a CBM-102 Bus Module integrator. Separation was achieved on the U Bondapak 125 A C18 10um column at room temperature. The samples were introduced through an injector valve with a 10 ul sample loop. Acetonitrile-water [1:1 v/v] was used as mobile phase, with flow rate 2 ml/minutes. pH was adjusted to 2.9 with phosphoric acid. UV detection was performed at 205 nm. The results obtained showed a good agreement with the declared content. Recovery values for cetirizine hydrochloride were 99.19 - 100.82%. The proposed method is reliable rapid, precise, selective and may be used for the quantitative analysis of cetirizine HCl, in presence of hyoscine butyl bromide as internal standard. The method was valid was for the determination in raw materials, bulk drug and formulations. The limit of quantification was 5 - 30 nano grams, while the limit of detection was 0.4 nano grams

In vitro availability of iomefloxacin hydrochloride in presence of essential and trace elements

Lomefloxacin hydrochloride is a third generation fluoroquinolone antibacterial agent having a broad spectrum of action against a wide range of Gram-positive and Gram-negative organisms. The in vitro availability studies of lomefloxacin were carried out in presence of essential and trace elements such as magnesium, calcium, chromium, ferric, ferrous, cobalt, nickel, copper, zinc and cadmium in simulated gastric juice, simulated intestinal juice and blood pH at 37oC using B.P 2003 dissolution test apparatus. It was observed that availability of lomefloxacin was depressed in presence of nickel and zinc in simulated gastric juice and in presence of Fe2+ in simulated intestinal juice, while many metals like magnesium, chromium, iron [both Fe2+ and Fe3+], cobalt, nickel, copper and cadmium depressed the availability of lomefloxacin at blood pH. Furthermore, the availability of lomefloxacin alone in simulated intestinal juice and at blood pH was reduced as compared to simulated gastric juice. The antibacterial activities of lomefloxacin in presence of these metal ions were observed and compared to control against six different microorganisms i.e., Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Bacillus fragilis and Streptococcus pneumonia by disc diffusion method to measure the inhibitory zone and MIC were determined by tube dilution method