ABSTRACT
The assessment of the degree or rate of cellular proliferation and cell viability is critical for the assessment of the effects of drugs on both normal and malignant cell populations. In the present study, a few novel 3-substituted derivatives of 2-indolinones were synthesized by condensation of substituted oxindole or isatin derivatives with appropriate aldehydes or primary aromatic amines respectively. The synthesized compounds were screened for their cytotoxicity against HT-29 [human colon adenocarcinoma cell line] and MCF-7 [human breast adenocarcinoma cell line] cells using short term cytotoxicity MTT assay protocol. A few derivatives with IC[50] < 10 microM were identified among them. The compound bearing 5-bromo substitution was the most potent derivative. Global physicochemical properties for compounds IVa-e and Va-h were calculated and the two most active compounds [IVa and IVb] showed similar Clog P-values