use of spectrophotometer in identification of bloodstains: normal haemoglobin and glycosylated hameoglobin

This study was attempted to apply an easy and low-cost method foridentification, differentiation of species of origin, and aging ofbloodstains. There were quite wide variations in the values of thealpha-ratio between bloodstains of different animal species and those of humanbeings. There was a significant negative correlation between themeans of the alpha-ratio and time in all the studied species extracted withboth ammonia and saline. This work showed an absorption spectrum, in thevisible region, of a minute speck of blood, which can prove that the substance under examination is blood. However, no accurate data could be given for thedifferentiation between animal and human blood, or between diabetic andnondiabetic human blood