Histological study on the effect of vitamin C on ischemia reperfusion injury in the adult rat ovary

Ovarian torsion may cause serious complications such as infertility in young women. Conservative management includes detorsion and reperfusion of the twisted segment. However, it may have local and systemic consequences due to production of large amounts of reactive oxygen species during reperfusion of ovaries. The present work aimed to study the possible histological and immunohistochemical changes due to ischemia-reperfusion injury in rat ovaries and the possible protective effect of vitamin C as an antioxidant. A total of 32 albino rats were divided into four groups. Group I was the control sham-operated group [either sham operated only, or with vitamin C administration]. In group II rats, ovarian ischemia was induced by torsion of the right adnexa. In rats of group III, 4 h of ischemia followed by reperfusion was performed. In rats of group IV, 4 h of ischemia was followed by 50 mg/kg vitamin C administration, which was injected intravenously, and then reperfusion was performed. Except for the ischemia group, all other groups were subdivided into two subgroups from which the right ovaries were surgically removed either after 5 h or after 2 weeks of starting the experiment. From the ischemia group ovarian samples were taken after 5 h only. Specimens were processed for paraffin sections and stained with H and E and with an immunohistochemical stain for apoptotic marker p53. Image analysis and statistical analysis of the obtained results were carried out. Severe vascular congestion, edema, hemorrhage, and increased P53 immunoreaction were detected in the ovaries after ischemia, which became less marked after reperfusion and considerably improved with vitamin C administration, especially after 2 weeks. Vitamin C treatment can help in protecting the ovaries from ischemia-reperfusion injury after detorsion

effect of mesenchymal stem cell therapy on ischemia-reperfusion-induced injury of the rat pancreas: a histological and immunohistochemical study

Ischemia-reperfusion [I/R] injury plays an important role in the development of acute pancreatitis; both ischemia and reperfusion contribute to tissue loss and organ dysfunction. I/R is also reported to be one of the reasons for the inflammatory reaction occurring in grafted tissue. Mesenchymal stem cells [MSCs] are multipotent cells capable of self-renewal and differentiation into various cell lineages. This study aimed to evaluate the role of MSC therapy on l/R-induced injury of the pancreas in albino rats. The present study was performed on three groups. Group I was the control group. Group II was the I/R group in which the pancreases of rats were exposed to ischemia for 30 min after which they were reperfused for 90 min; these rats were then sacrificed 1 week [group I la] and 4 weeks [group lib] after reperfusion. Group III was the stem cell-treated group in which rats were exposed to I/R and then injected intravenously with MSCs; they were then sacrificed 1 week [group Ilia] and 4 weeks [group Illb] after reperfusion. Pancreatic sections were stained with Hand E, CD105, and insulin. Results were statistically analyzed. I/R caused changes in the form of cellular vacuolations and apoptotic changes involving the pancreatic acini. Immunohistochemical staining for insulin was markedly decreased, becoming almost absent by the fourth week. Treatment with MSCs was associated with PKH 26-labeled cells within the exocrine and endocrine portion of the pancreas. Also, CD105-positive cells were detected between the acini and within the stroma in between. The cells in the treated subgroups restored their normal appearance and regained positivity for insulin immunoreactivity