ABSTRACT
The study investigated the protective effect of grape seed and skin extract [GSSE] against doxorubicin-induced renal toxicity in healthy rats. Animals were treated with GSSE or not [control], for 8 days, administered with doxorubicin [20mg/kg] in the 4th day, and renal function as well as oxidative stress parameters were evaluated. Data showed that doxorubicin induced renal toxicity by affecting renal architecture and plasma creatinine. Doxorubicin also induced an oxidative stress characterized by an increase in malondialdehyde [MDA], calcium and H2O2 and a decrease in catalase [CAT] and superoxide dismutase [SOD]. Unexpectedly doxorubicin increased peroxidase [POD] and decreased carbonyl protein and plasma urea. Treatment with GSSE counteracted almost all adverse effects induced by doxorubicin. Data suggest that doxorubicin induced an oxidative stress into rat kidney and GSSE exerted antioxidant properties, which seem to be mediated by the modulation of intracellular calcium