Raised 17-hydroxyprogesterone levels in congenital adrenal hyperplasia

Congenital adrenal hyperplasia [CAH] refers to autosomal recessive diseases resulting from deficiency of enzymes involved in the production of cortisol by the adrenal glands. This study was designed to determine the frequency of suspected congenital adrenal hyperplasia patients by evaluating the laboratory data of blood 17-OHP. The study was conducted at Chemical Pathology Section of Department of Pathology at the Aga Khan University, Karachi. The basic demographic data of 2282 subjects was recorded, screened for blood 17-OHP levels from January 2007 to December 2010. A cutoff of >/= 4 ng/ml was considered as suggestive of CAH. The results showed 17-OHP levels >/= 4 ng/ml were found predominantly among infants [14.4%] and in females [18.2%]

Agreement between serology and histology for detection of helicobacter pylori infection

To determine the percentage agreement between serology and histology for detection of Helicobacter [H.]pylori infection. Cross-sectional analytical study. Department of Pathology and Microbiology, The Aga Khan University and Hospital, Karachi, from January to December 2009. Fifty subjects were selected by non-probability purposive sampling from laboratory data who had serological testing of H. pylori IgG antibody, prior to histological evaluation of endoscopic gastric or/and duodenal biopsies. Serological Quantification of H. pylori IgG was carried out with HpG screen ELISA kit [Genesis Diagnostics, UK], using an enzyme linked immunosorbent assay for detection of IgG antibodies against H. pylori. Manufacturer's recommended cutoff value was used and results were considered positive when greater than 7 U/ml. For histological diagnosis, an expert histopathologist characterized the presence of spiral bacteria in the mucosal layer or the surface of epithelial cells on microscopic examination, as a positive test. An agreement of 0.72 was found by Kappa statistics between serology and histopathology results and a good diagnostic accuracy [86%] of serological testing was observed for the diagnosis of H. pylori infection. A substantial agreement was found between serology and histopathology results to detect the H. pylori infection. Laboratory-based serologic testing using ELISA technology to detect IgG antibodies is inexpensive, noninvasive and convenient method to detect the H. pylori infection in primary care setting.