ABSTRACT
Introduction: the present study was carried out to evaluate the effect of the non-steroidal anti-inflammatory drug diclofenac sodium [DS] on the fetuses of albino mice from the morphological and skeletal points of view
Material and methods: sixty adult pregnant female mice were used in the present study. They were allocated into 6 groups [10 mice each]. The first two groups served as control and were injected intraperitoneally [i.p.] with the solvent of the drug, and the 3rd and 5th groups were treated with 1.5 and 3mg/kg body weight of diclofenac sodium for 6 days [ gestation days 1-6 ], respectively ; the 4th and 6th groups were treated with 1.5and 3mg/kg body weight of the drug for 8 days [ gestation days 7-14], respectively
Results: the morphological examination of the fetuses of treated groups showed conspicuous decrease in the average body weight and body length in all treated groups. The fetuses maternally treated with the drug showed noticeable external morphological malformations and their skeletons exhibited mild retardation in skeletal elements
In conclusion: the non-steroidal anti-inflammatory drug diclofenac sodium had exerted marked morphological malformations and mild skeletal alterations in mice fetuses maternally treated during different periods of gestation
ABSTRACT
A hypothetical role of mast cells, endothelial cells and fibroblasts, in the pathophysiology of scleroderma has been proposed. The present study aimed at the illustration of the ultrastructural changes of these cells, in particular, in the skin of patients with scleroderma. In the following work, electron microscopic examination demonstrated a spectrum of degranulated mast cells as well as different grades of cell-activation evidenced by presence of hypodense granules with increased cytoplasmic organelles such as rough-surface endoplasmic reticulum [RER], polysome and mitochondria. The fibroblasts showed numerous dilated RER, intra and extracytoplasmic filaments. Some fibroblast - like cells were seen ingesting some mast cell granules. Evidences of endothelial injury were detected in the form of thickening and stratification of basal lamina and swollen endothelial cells