ABSTRACT
As acid soils of Odisha have been facing a major constraint in phosphorus availability, application of native P solubilizing bacteria could be promising as well as ecofriendly step towards sustainable P availability for crop growth and development. To address the problem of P availability in acid soil of Bhubaneswar, Odisha, rhizosphere soil samples (pH ? 5.50) with rice – pulses (green gram/black gram) cropping system were collected and phosphate solubilising bacteria were isolated. In vitro characterization of the PSB isolates were conducted with calcium, aluminium and iron phosphates to recover soluble P. All the five strains i.e. Bacillus cereus BLS18 (KT582541), Bacillus amyloliquefaciens CTC12 (KT633845), Burkholderia cepacia KHD08 (KT717633), Burkholderia cepacia KJR03 (KT717634), Burkholderia cepacia K1 (KM030037) could solubilize Ca3(PO4)2, AlPO4, FePO4, and Fe3(PO4)2. Higher recovery of soluble P was with Ca3(PO4)2 while the least was with AlPO4. All the strains exibited a trend similar with respect to P recovery i.e. Ca3(PO4)2 > FePO4 > AlPO4 > Fe3(PO4)2. B. amyloliquefaciens CTC12 was most efficient in solubilizing calcium and iron phosphates whereas B. cepacia KHD08 recovered maximum P with aluminium phosphate. All the inorganic salt fortified mediums showed a significant decline in pH which necessitated the identification of compounds present in the mediums. Organic acids viz; acetic, citric, gluconic, lactic, malic, succinic, tartaric acids in the mediums were identified by HPLC. Tartaric acid was only found in the mediums supplemented with AlPO4. B. amyloliquefaciens CTC12 and B. cepacia KHD08 showed promising results in in vitro analysis of P solubilization. The present study is focused on problematic acid soils where phosphorous is unavailable and mostly fixed with aluminium and iron ultimately making it unavailable for the crops to take up. This leads to unbalanced and frequent use of chemical fertilizer. Hence the study is a significant attempt to characterize native PSBs with capacity to solubilize Al-P and Fe-P
ABSTRACT
Malignant peritoneal mesothelioma is a rare lethal malignancy of the serosal membranes of the peritoneum. The pathogenesis and association is strongly related with industrial pollutants asbestos, but less than pleural mesothelioma. Symptoms are nonspecific and related to the tumor spread within the abdominal cavity. CT scan is the investigation of choice and mostly disease is discovered incidentally on routine imaging. Diagnosis is confirmed on histopathology as well as immunohistochemical analysis of markers. The mainstay of treatment is cytoreductive surgery with Hyperthermic intraperitoneal chemotherapy. Here we present a very unusual case of malignant peritoneal mesothelioma diagnosed on routine evaluation of a 62 year old male admitted in emergency for obstructed inguinal hernia. Introduction: Malignant peritoneal Mesothelioma MPM is a very rare malignancy of the abdominal cavity. Mesotheliomas usually originate from the serosal membrane of different body cavities. Pleura is most commonly affected by mesothelioma followed by peritoneum and also other cavities pericardium and tunica vaginalis testis.10 to 30% of all mesothelioma affects peritoneum. Malignant peritoneal mesothelioma is a highly lethal malignant tumor of peritoneum and its pathogenesis is strongly related with industrial pollutant asbestos exposure. Diagnosis is difficult in most of the cases because of its nonspecific presentation and detected on routine abdominal imaging or Surgery.
ABSTRACT
Background. The aim of the study is to analyze the pattern of mandibular fracture in eastern Indian population. Methods: A retrospective study of 92 computed tomographic examination imaging data showing mandibular fractures during the period from January 2016 to June 2019 were retrieved from radiology data storage and was evaluated. Data were analyzed statistically. Result-The study revealed 83 (90.2%) male and 9 (9.8%) female cases with mean age 34.3 and male female ratio 11.8/1. The most common cause of injury was road traffic accident. The most common fracture sites were parasymphyseal location (92%) followed by mandibula body fracture. The mandibular fractures were treated by open reduction and internal fixation. Conclusion: The most common cause of mandibular fracture in eastern Indian population was RTA. The parasymphyseal fracture is the most common type of mandibular fracture.
ABSTRACT
Background: Prospective magnetic resonance imaging (MRI) of orbits in healthy paediatric population is difficult as it involves sedation or general anaesthesia in most of the cases. The data of normal value of healthy paediatric optic nerve and optic nerve sheath diameter is also scant in literatures for Indian paediatric population. The normal data will help in defining abnormal optic nerve such as congenital optic nerve hypoplasia, optic nerve atrophy and distended optic nerve sheath in raised intracranial pressure. AIM: The aim of our retrospective study is to define the normal value of optic nerve and optic nerve sheath diameter in MRI of paediatric Indian population. MATERIAL AND METHODS:190 MRI studies of paediatric brain with normal radiology reports and no history of raised intracranial pressure were retrospectively reviewed by two senior radiologists using T2 weighted axial brain images. RESULT: The mean optic sheath diameter and optic nerve diameter of 380 eye balls were 4.746 and 2.59 mm respectively. The age wise distribution of optic sheath diameter and optic nerve diameter were 4.08±0.019, 2.05±0.608 in 0-2-year age group, 4.83±0.575, 2.54±0.456 in 3-6-year age group, 4.789±0.553, 2.704±0.447 in 7-12-year age group, and 4.82±0.567, 2.65±0.412 in 13-18 years age group in mean (mm)±standard deviations respectively. Three hundred seventy-one (97%) optic sheath measurement of the 380 eye balls was below the threshold of 6 mm and about 352 (92%) optic nerve measurements out of 380 globes were having optic nerve thickness above 2 mm. This normative data will help the ophthalmologist, clinician, neuro-ophthalmologist and radiologists for better quantitative evaluation of pediatric patient with vision abnormality.
ABSTRACT
During the efficient genetic transformation of plants with the gene of interest, some selectable marker genes are also used in order to identify the transgenic plant cells or tissues. Usually, antibiotic- or herbicide-selective agents and their corresponding resistance genes are used to introduce economically valuable genes into crop plants. From the biosafety authority and consumer viewpoints, the presence of selectable marker genes in released transgenic crops may be transferred to weeds or pathogenic microorganisms in the gastrointestinal tract or soil, making them resistant to treatment with herbicides or antibiotics, respectively. Sexual crossing also raises the problem of transgene expression because redundancy of transgenes in the genome may trigger homology-dependent gene silencing. The future potential of transgenic technologies for crop improvement depends greatly on our abilities to engineer stable expression of multiple transgenic traits in a predictable fashion and to prevent the transfer of undesirable transgenic material to non-transgenic crops and related species. Therefore, it is now essential to develop an efficient marker-free transgenic system. These considerations underline the development of various approaches designed to facilitate timely elimination of transgenes when their function is no longer needed. Due to the limiting number of available selectable marker genes, in future the stacking of transgenes will be increasingly desirable. The production of marker-free transgenic plants is now a critical requisite for their commercial deployment and also for engineering multiple and complex trait. Here we describe the current technologies to eliminate the selectablemarker genes (SMG) in order to develop marker-free transgenic plants and also discuss the regulation and biosafety concern of genetically modified (GM) crops.