ABSTRACT
Escherichia coli isolates from different samples of droppings collected from the different farms of broiler and local poultry were characterized to confirm the virulence. E. coli isolates recovered from different farms were examined for presence of genes encoding patho groups such as shiga like toxin producing Escherichia coli (STEC), (stx1/stx2) by Duplex PCR and then analysed the PCR Amplicons by AGE. The three and four isolates of E. coli recovered from local and boiler poultry were STEC because of presence of the stx1 and stx2 genes. Presence of stx1 and stx2 genes clearly indicated these as prime cause of pathogenecity. Further, demonstration of STEC in poultry becomes a public health concern, as poultry are potential reservoir of such agents, which may cause extra intestinal diseases like haemolytic uremic syndrome and thrombocytopenic purpurea by stx1 &2 and these toxins kill vascular endothelial cells.
ABSTRACT
PCR detection of ‘stx 1’ and ‘stx 2’ toxigenic genes in multiple antibiotic resistant Escherichia coli population and Phenotypic detection of ESBL producing Escherichia coli isolated from traditional local variety of poultry was conducted The study revealed that out of 30 samples collected 20 were positive for E.coli All the 20 isolates were subjected for the detection of 2 toxin genes (stx1 & stx2 genes) by PCR. Only 3 isolates (L3, L4 & L5) gave rise to PCR product against its specific primers of stx1. All the isolates were negative for the presence of stx2 gene. Thus 15% of E.coli population under this study is potential isolates and indicates that healthy retail poultry birds are the carrier of E.coli pathogenic strains. The poultry birds may therefore, be considered as a major reservoir of E.coli.