Reporte de un probable caso de Hemoglobina S / Talasemia Beta / Report of a probable case of Hemoglobin S / Beta Thalassemia

We report the case of a patient with Hemoglobin S / Thalassemia. It is feasible to recognize this infrequent disease by its clinical presentation and the aid of clinical laboratory. On this patient, the diagnosis was established based on the clinical findings, hematological evaluation (with careful observation of the red cell morphology and reticulocyte count) and electrophoretic analysis of hemoglobin. We discusse the physiopathology, clinical manifestations, treatment and alternative of prevention of this disease.

Determinación de factores de virulencia asociados a Escherichia Coli Enterohemorrágica en cepas peruanas aisladas entre 1999-2001 / Determination of virulence factors related to Escherichia Coli enterohemorragic Peruvian strain isolated between 1999-2001

En el presente estudio se intentó detectar la presencia del gen de toxina en cepas locales de Escherichia Coli serológicamente relacionados a la catergoria enterohemorrágica, caracterizando además un aislamiento reportado como serotipo 0157:H7 procedente de la ciudad de Tacna (cepa Tacna 410), mediante la reacción en cadena de la polimerasa (PCR) y secuenciamiento. Los resultados confirmaron la presencia del gen de la toxina shiga sólo en la cepa Tacna 410, obteniéndose una identidad del 100 por ciento entre la secuencia nucleótida del gen de la copa Tacna 410 y secuencias reportadas de la toxna shiga de tipo II en el Genebank. Asimismo, se detectó en la cepa Tacna 410 propiedades hemolíticas y el gen eae asociado al fenómeno de attaching and effacing, características de una típica cepa de ECEH.

We tried to detect the Shiga gene in local Escherichia Coli strains serologically related the enterohemorragic category. At the same time, using polymerase chain reaction (PCR) and sequencing, we characterized a strain confirmed as E. Coli 0157:H7 serotype, which was isolated in Tacna (a city in southern Peru) (Tacna 410 strain). Our results confirmed the presence of the Shiga toxin gene only in E. coli strain Tacna 410, and we found 100 percentage identify between the sequence from the amplified gene and reference sequence for type II Shiga toxin in the gene bank. We also detected in the Tacna 410 strain hemolytic properties and the eae gene, which is associated to attaching and effacing lesions, typical features of EHEC, strains.