ABSTRACT
OBJECTIVE@#To study the recombination events among enterovirus strains and the development of specific primers for the detection of enteroviruses in environmental samples.@*METHODS@#Nucleotide sequence analysis of enteroviruses deposited in the international database GenBank (www.ncbi.nlm.nih.gov/Genbank) was conducted to develop specific primers for the detection of these viruses. The specificity and sensitivity of the method were tested using coxackievirus B3 strain Nancy, environmental isolate of human hepatitis A virus and human rotavirus strain WA. Seventy sewage samples were analyzed.@*RESULTS@#Enterovirus genome was detected in all positive samples. The genome of enterovirus was not detected in negative samples. The level of detection of these viruses was 10(2) TCID(50)/mL.@*CONCLUSIONS@#The development of new primers is an important issue for the detection of enteroviruses in the environment and the assessment of risk factors to human health.
Subject(s)
Humans , 5' Untranslated Regions , Genetics , Biological Evolution , Enterovirus B, Human , Genetics , RNA , Genetics , RNA, Viral , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, RNA , Sewage , VirologyABSTRACT
An acquired factor VII deficiency was identified in a 63-year-old man with bronchogenic carcinoma. Initial studies indicated a normal activated partial thromboplastin time and a prolonged prothrombin time. The factor VII level was 6%. No evidence of a factor VII inhibitor or inactivator was demonstrable. However, on account of the initial normal laboratory test of emostases, the partial correction of the prothrombin time with 50% normal plasma in vitro and the family history, the congenital deficiency in factor VII was ruled out. Whatever the mechanism involved, this factor VII deficiency was related to malignancy