ABSTRACT
Infertility has become nowadays not only a medical, bur a Social problem as well. Infertility in female is caused by various factors. Tubal disease is responsible for 25-35% of female infertility. Pelvic inflammatory disease [PID] is the most common cause of tubal disease
Aim of the study: determine the frequency of tuberculosis and C. trachomatis in patients with tubal factor infertility to develop a laboratory strategy for identification of the most common causes of tubal factor infertility [M. tuberculosis and C. trachomatis] in our locality with respect to laparoscopic finding. Patients and Methods: this study was performed on ninety women with primary infertility and abnormal hysterosalpingography as studied group and 30 infertile women [with polycystic ovary as the only cause of infertility] and normal hysterosalpingography as a control group. Mycobacterium tuberculosis IgG and of C. trachomatis IgG antibodies were performed by enzyme linked immunoassay [ELlSA]. Polymerase chain reaction [PCR] was performed .for detection mnpt64 gene of M. Tuberculosis and C. trachomatis plasmid DNA
Results: in patient group, M. Tuberculosis and C. trachomatis IgG antibodies showed positive results in 72 [80%] and 13 patients [81.1%] respectively. In control group they were detected in 23 [76.7%] and 5 [16.6%] respectively. PCR for mpt64 gene of M. tuberculosis was detected in peritoneal fluid of 17 patients [18.89%] af7d and C. trachomatis plasmid DNA were detected 5 patients [5.56%] respectively
Conclusions: tuberculosis is one of the major etiological factors for female infertility in Egypt and the use PCR for nzpt64 gene o f M tuberculosis plays on important role in diagnosis of genital tuberculosis with results comparable with laparoscopy and histopathology. Detection of C. trachomatous 1gG antibodies by ELlSA can play a significant role in detection of C. trachomatis in infertile women