Refadin as humoral immunomodulant in a lapin models

Serial subtheraputic concentrations of Refadin[R] that ranged from 0-6 mg/m/kg were made in 2v. egg albumin antigen. Two protocols for immunopriming rabbits were attempted, as oral and multiinjection. Same concentrations were incorporated in vitro in the diluting buffur of immune and hyper-immunesera of anti EA Specificity. In each case immunomodulation was scored as enhancement of anti EA titres or reduction in anti EA titres using microtitrehaermagglutination assay. It has been found that; the priming rout, the nature of adjuvant used, the nature of antibody as serum or mucosal and the drug concentration as well as the approach nature as in vitro or in vivo, were the factors affecting the humoral immune modulation. This immunomodulation was in form of immunosuppressive type, antigen independent, dose dependent in which the antigen was T independent, B cell mitogen. The dose - response relation was of simple linear negative type. Based upon this results drug induced immunosuppression in tuberculus patients is quite possible

herbicide 2-4-D as a lapin humoral ecoimmunotoxicant

Several concentration of 2-4-D that ranges from 0.72 to 7.2 mg / ml / kg, were made in the aqious 2V% solution of egg albumin and used for priming rabbit through multisite injection protocol. Same concentration, however, were also made in the diluting buffer used for titration of anti EA in hyperimmune and immunesera invitro. The immunomodulation was scored as either increase or decrease in anti EA antibody titres than those of control.The dose-response curve was of simple linear negative type. Thus, 2-4-D is being classified as environmental immunotoxicant, nonantigen specific immunomodulant and of suppressive mode of action. It is a B cell immunosuppressent. It is being considered as occupational hazard in working farmers