ABSTRACT
The aim of this study was to evaluate the effect of banana leaf extract on the quality and shelf life of rainbow trout compared to plastic bags at freezing temperature for 40 days. For evaluating this propose, the antioxidant activity of banana leaf extract was assessed. In addition, the shelf life of fish filets was determined by measuring thiobarbituric acid (TBA) and pHof fish. The banana leaves extract showed the highest content of vitamin E (5.8 ± 0.61 mg /g) and carotenoids (12.8 ± 0.1 mg /g). The potential of Cu (II) reduction the extract was 1.76 ± 0.09. The magnitude of modification in TBA and pH of the packed fish with banana leaves were less than the control samples. The present study demonstrated that the use of banana leaf extract will retard lipid oxidation in fish. fillet during freezing storage that may due to its strong antioxidant properties.
O objetivo deste estudo foi avaliar o efeito do extrato de folhas de bananeira sobre a qualidade e vida de prateleira da truta arco-íris comparada a sacolas plásticas na temperatura de congelamento por 40 dias. Para avaliar essa proposta, foi determinada a atividade antioxidante do extrato de folhas de bananeira. Além disso, a vida de prateleira dos filés de peixe foi determinada medindo o ácido tiobarbitúrico (TBA) e o pH do peixe. O extrato de folhas de bananeira apresentou o maior teor de vitamina E (5,8 ± 0,61 mg/g) e carotenóides (12,8 ± 0,1 mg/g). O potencial de redução de Cu (II) no extrato foi de 1,76 ± 0,09. A magnitude da modificação no TBA e pH do peixe embalado com folhas de bananeira foi menor que as amostras controle. O presente estudo demonstrou que o uso de extrato de folhas de bananeira é capaz de retardar a oxidação lipídica no filé de peixe durante o armazenamento de congelamento, devido às suas fortes propriedades antioxidantes.
Subject(s)
Plant Extracts , Oncorhynchus mykiss , Musa , Date of Validity of Products , Antioxidants , Product Packaging , FreezingABSTRACT
INTRODUCTION: Honey is a common household product with many medicinal uses described in traditional medicine. Only recently has its antioxidant properties and preventive effects against disease been highlighted. Chrysin is a natural flavone commonly found in honey that has been shown to be an antioxidant agent. In this study, we investigated the antiproliferative and apoptotic effects of honey and chrysin on cultured human prostate cancer cells. METHODS: Cells were cultured in RPMI medium and treated with different concentrations of honey and chrysin for three consecutive days. Cell viability was quantitated by the 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The percentage of apoptotic cells was determined by flow cytometry using Annexin V-fluorescein isothiocyanate. RESULTS: The MTT assay revealed that both compounds had an antiproliferative effect on PC-3 cells in a dose- and time-dependent manner. The IC50 values for honey and chrysin against PC-3 cells were 2.5 percent and 24.5 percent after 48 h and 1.8 percent and 8.5 percent after 72 h, respectively. Chrysin induced apoptosis in PC-3 cells, as determined by flow cytometry. CONCLUSION: Our results suggest that honey has anti-proliferative effects on prostate cancer cells and the effects are mainly due to chrysin. Therefore, chrysin may be a potential compound for both cancer prevention and treatment. Further in vivo investigation is needed to support the use of chrysin in cancer therapy.