Effect of three types of denture cleansers on the streptococcus mutans count in saliva

This study was conducted to detect the effect of three types of commercially available denture cleansers clinically on the oral mucosa and bacteriologically on saliva. Forty edentulous patients free from any oral or systemic diseases were selected in this study. Conventionally constructed dentures were delivered. At the beginning of the study the clinical condition of the mucosa was recorded. Also samples of saliva were taken, diluted and cultured on blood agar medium to count the total colony forming units, and on mitis sucrose bacitracin to count the streptococcus mutans in the samples. The patients were divided into four groups each of ten patients. For every group denture cleanser is prescribed and patients are advised to use it according to manufacturer's instructions, any other method of cleansing was prohibited. The fourth group is a control group without use of any cleanser. Patients were recalled every month for checkup of the oral mucosa and inspection of the dentures. Samples of saliva were taken before denture insertion, one month after denture insertion, [before denture cleanser use], end of the second, fourth and seventh month, these samples were diluted, and cultured, also the clinical condition of the mucosa was recorded and statistically analysed. The results indicated that chemical cleansing of dentures decreased the total number of colony forming of microorganisms and the number of streptococcus mutans. The condition of the mucosa was improved with the decrease of the oral microorganisms. Chemical cleansers containing peroxide are better than those containing sodium hypochlorite. No difference between effervescent tablets and non effervescent powder of the peroxide type was found

Exfoliative cytology of the oral mucosa under free end saddles recorded by two different impression techniques, muco-static and muco-compressive

Exfoliative cytology of the oral mucosa under distal- extension bases recorded by two different impression techniques: mucostatic and mucocompressive was investigated in this study. Eleven, patients were selected and divided into two groups [five in each group as one did not continue to the end of the study]. Each patient in the two groups received a mandibular distal-extension removable partial denture with an opposing complete maxillary denture. The mandibular partial dentures in the first group were constructed from mucostatic impressions, while, in the second group from mucocompressive impressions. All other steps of denture construction were made in a standard manner for all patients. Cytologic smears of oral mucosa under distal-extension bases were collected, fixed, stained with Papanicolaou's stain and then examined microscopically to calculate the Keratinization index. This cytologic examination was done : at the time of delivery and after : one mouth, two months and three months of denture wear. At each time interval, buccal, crestal and lingual smears were collected at the first molar region of the right side. The three smears were examined and then a mean Keratinization was calculated. Data of the cytologic examination of all patients were collected and statistically analyzed. It was found that Keratinization Index of the oral mucosa was significantly reduced under lower distal-extension bases recorded by either: mucostatic or mucocompressive technique. Maximum reduction was detected after one month of denture wear. The reduction in keratinization Index was significantly more under dentures constructed with mucocompressive impression technique than with mucostatic impression technique