Study on microbial causes of corneal ulcer and antibiotic sensitivity tests

A microbiological study of 120 patients with corneal ulcers due to bacterial and fungal infections was performed. Positive cultures were obtained in 67 percent of all samples. 20 patients with negative cultures had received antimicrobial treatment prior to sampling. Staphylococcus epidermis was the commonest bacteria isolated from central ulcers followed by streptococcus pneumonie while in marginal ulcers staphylococcus aureus predominated. Aspirgillus fumigatus was the most common fungus isolated from central ulcers while in marginal ulcers, aspirgillus Niger was more common. Antibiotic sensitivity tests revealed that most gram positive isolates were sensitive to bacitracin, clindamycin and fucidin. As concerns gram negative isolates, almost all pseudomonas aeruginosa strains [the commonest gram negative isolate] were susceptible to amikacin, gentamycin and polymyxin B

relationship between enterotoxin production and serotypes of E coli

Two-hundred strains of E. coli were isolated from children suffering from diarrhoea. Using antisera against the known serotypes of enteropathogenic E. coli 58 strains out of the total 200 [29%], could be typed and were considered as enteropathogenic strains. The rest were non-typable and were considered as nonenteropathogenic strains. Thirty strains of enteropathogenic E. coli [typable] as well as thirty non-enteropathogenic strains [non-typable] were tested for toxigencity by the infant mouse test. The results showed that enterotoxin was produced by 23 enteropathogenic strains [76.6%] and by 17 non-enteropathogenic strains [56.6%]. Thus toxigenicity was proved to be not restricted to enteropathogenic serotypes, but also to non-enteropathogenic strains. These findings reduce the importance of the routine screening of E. coli for detection of enteropathogenic serotypes

Detection of hepatitis B antigen in blood donors

Detection of HB[s] Ag was attempted in this work with both paid and volunteer blood donors as well as with patients already diagnosed in the active phase hepatitis. Counter immunoelectrophoresis [CIEP] and agarose gel immunodiffusion were attempted to compare their sensitivities. CIEP method was more sensitive and more rapid than the gel immunodiffusion. The incidence of carrier state was 3.67% among blood donor population by using CIEP. Paid donors were found to be more susceptible for HB[s] Ag persistent antigenaemia than volunteer donors

Studies on the effect of some inhalational anaesthetic agents on the immune response of experimental animals

The humeral immune response was depressed to a variable degree by nitrous oxide, halothane, ether and also by exposure to pure oxygen. In this respect, halothane was the most noxious agent while pure oxygen was the most innocent. In between lied ether and nitrous oxide. The cellular immune response was significantly depressed by halothane and ether while N[2]0 and oxygen had no significant effect. Diethyl ether was the most potent agent in depressing the DHR while halothane was the most potent agent in depressing the anti-tumour immunity. The mean survival times was significantly decreased by halothane only