ABSTRACT
During the present study an effort has been made to determine the sensitivity of clinical isolates to complish this task, ninety clinical isolates of Escherichia coli [n-41], Klebsialla spp. [n-12], Proteus spp [n-6], Pseudomonas aeruginosa [n-11], and Staphylococcus aureus [n-20] were collected from different pathological laboratories and medical center in Karachi. An in vitro study of these clinical isolates were carried out by ICLS reference Disk diffusion [Kirby-Bauer] method, using Cephradine and Cefotaxime. An attempt has been made to measured the Zone if inhibition produced by Cephradine and Cefotaxime against these clinical isolates. Results indicated that both Cephradine and Cefotaxime has excellent in-vitro antibacterial activity, but Cefotaxime had relatively broad spectrum of activity against most of clinical isolates tested its mean that Cefotaxime is more effective against Gram-positive and Gram-negative bacteria. On the basis of present study it can be evaluated that Cephradine and Cefotaxime were two very good antibacterial agent in the field of antimicrobial Chemotherapy but different clinical isolates had started to develop resistance to these antibiotics, which is shocking for prescriber because these two cephalosporins are massively prescribe for most of infections in our country