ABSTRACT
The study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of Fasciola gigantica experimentally infected rabbits. Copro Hyper Immune Serum [HIS] and Excretory-Secretory Hyper Immune Serum [ES HIS] antigens were used in a sandwich ELISA for the detection of F. gigantica antigens in feces of 12 rabbits experimentally infected with different doses of F. gigantica encysted metacercariae [EMC] [10, 25 and 30 EMC]. The relation between time of appearance of coproantigens in feces and anti-Fasciola antibodies in serum was evaluated. The earliest diagnostic coproantigen was recorded at 21[st], 25[th] and 28[th] day post-infection [p.i.] in groups of rabbits infected with 30, 25 and 10 F. gigantica EMC respectively. Both HIS and ES HIS were able to detect coproantigens in feces of rabbits infected with 30 EMC at day 21 p.i. The appearance of F. giganticacoproantigens in feces of infected rabbits was concurrent to the appearance of anti-Fasciola antibodies in blood [3[rd] week p.i.]. However, coproantigen has specific ability for direct assessment of active infection with minimal cross-reaction with other heterologous parasitic infections. The findings hold promise for a more accurate diagnostic technique in the near future for suspected Fasciola infection
ABSTRACT
Spectrophotometric, conductimetric and potentiometric methods are developed for the determination of diflunisal in pharmaceutical preparations. The first method was based on the formation of a yellow colored complex between diflunisal and uranyl acetate in alcoholic medium. The optimum reaction conditions, spectral characteristics, conditional stability constant and composition of the soluble complex have been established. The method permits the determination of diflunisal over a concentration range20-l45 microg/ml, with molar absorptivity 1.66x10[3] L Mol [-1] cm [-1] and Sandell sensitivity 0.151 microg cm [-2].The second method allowed the determination of the drug over a wide range [2.5-25 mg] through the eonductimetric titration of diflunisal with uranyl acetate solution. The third method depends on the direct potentiometric titration using uranyl acetate as a titrant in alcoholic medium. The characteristic curve break occurs at molar ratio [reagent/ drug 1:1]. Statistical analysis of the result: has been carried out revealing high accuracy and good precision. The suggested procedures could be used for the determination of the drug in pure and dosage forms
ABSTRACT
This study was carried out to develop a conductimetic titration method for determination of cyproheptadine hydrochloride [CpCl], cetirizine dihydrochloride [CetCl2] and terbinafme hydrochloride [TerCl] with manganese and zinc thiocyanate complexes [Mn [SCN]4][2-] and [Zn [SCN]4][2-] as titrants through ion-associate complex formation. The solubility products of the formed ion-associates were determined. The method was found to be highly accurate and precise having a relative standard deviation of less than 1.0%. Also it was shown that the method could be successfully applied to assay of commercial pharmaceuticals containing the above-mentioned drugs and the results are favorably comparable to the official methods