ABSTRACT
Toxocariasis is a soil-transmitted helminthozoonosis due to infection of humans by larvae of Toxocara canis. The disease could produce cognitive and behavioral disturbances especially in children. Meanwhile, in our modern era, the incidence of immunosuppression has been progressively increasing due to increased incidence of malignancy as well as increased use of immunosuppressive agents. The present study aimed at comparing some of the pathological and immunological alterations in the brain of normal and immunosuppressed mice experimentally infected with T. canis. Therefore, 180 Swiss albino mice were divided into 4 groups including normal (control) group, immunocompetent T. canis-infected group, immunosuppressed group (control), and immunosuppressed infected group. Infected mice were subjected to larval counts in the brain, and the brains from all mice were assessed for histopathological changes, astrogliosis, and IL-5 mRNA expression levels in brain tissues. The results showed that under immunosuppression, there were significant increase in brain larval counts, significant enhancement of reactive gliosis, and significant reduction in IL-5 mRNA expression. All these changes were maximal in the chronic stage of infection. In conclusion, the immunopathological alterations in the brains of infected animals were progressive over time, and were exaggerated under the effect of immunosuppression as did the intensity of cerebral infection.
Subject(s)
Animals , Female , Male , Mice , Brain/pathology , Disease Models, Animal , Gene Expression Profiling , Histocytochemistry , Immunocompromised Host , Immunohistochemistry , Interleukin-5/genetics , Parasite Load , Toxocara canis/immunology , Toxocariasis/immunologyABSTRACT
Toxocariasis is a soil-transmitted helminthozoonosis due to infection of humans by larvae of Toxocara canis. The disease could produce cognitive and behavioral disturbances especially in children. Meanwhile, in our modern era, the incidence of immunosuppression has been progressively increasing due to increased incidence of malignancy as well as increased use of immunosuppressive agents. The present study aimed at comparing some of the pathological and immunological alterations in the brain of normal and immunosuppressed mice experimentally infected with T. canis. Therefore, 180 Swiss albino mice were divided into 4 groups including normal (control) group, immunocompetent T. canis-infected group, immunosuppressed group (control), and immunosuppressed infected group. Infected mice were subjected to larval counts in the brain, and the brains from all mice were assessed for histopathological changes, astrogliosis, and IL-5 mRNA expression levels in brain tissues. The results showed that under immunosuppression, there were significant increase in brain larval counts, significant enhancement of reactive gliosis, and significant reduction in IL-5 mRNA expression. All these changes were maximal in the chronic stage of infection. In conclusion, the immunopathological alterations in the brains of infected animals were progressive over time, and were exaggerated under the effect of immunosuppression as did the intensity of cerebral infection.
Subject(s)
Animals , Female , Male , Mice , Brain/pathology , Disease Models, Animal , Gene Expression Profiling , Histocytochemistry , Immunocompromised Host , Immunohistochemistry , Interleukin-5/genetics , Parasite Load , Toxocara canis/immunology , Toxocariasis/immunologyABSTRACT
The study included 151 patients in 3 groups: 61 patients [group I] with schistosomal hepatic fibrosis and ascites including 25 with [group I a] and 36 without prolonged or recurrent diarrhoea [group I b], 60 patients [group II] with liver cirrhosis and ascites including 25 with [group II a] and 35 without prolonged or recurrent diarrhoea [group II b] and 30 patients [group III] with prolonged or recurrent diarrhoea without liver disease. Twenty five healthy subjects from their relatives were used as control. All came from a rural area near Tanta city and were selected to study the relation of schistosomal hepatic fibrosis or cirrhosis to Cryptosporidium parvum infection and the possible role of environment in this rural community. Stool examination using safranine - methylene blue and direct immunofluorescent stains for oocysts and serum examination by indirect immunofluorescent technique for antioocyst antibody levels. The results showed significant difference between group I a versus I b, II a versus II b, I a + II a versus lb + II b, I a + II a versus III and all subgroups versus control but insignificant between lb, II b and lb + II b versus III and I versus II and insignificant difference between methods of stool and serum examination as regards positivety. Patients positive for Cryptosporidium are usually debilitated and having watery diarrhoea and sometimes abdominal cramps. Patients and control gave history of contact with domestic animals. It may be concluded that patients with liver fibrosis or cirrhosis are liable to Cryptosporidium infection which is higher in those associated with severe watery diarrhoea and the environment has a role in infection of potentially immune suppressed patients