ABSTRACT
Background and Objectives: Helicobacter pylori [H. pylori] infection is cause of several gastrointestinal diseases in humans. Virulence genes of H. pylori are associated with severity of disease and vary geographically. The aim of present study was to detect H. pylori in formalin-fixed paraffin-embedded [FFPE] tissues and further investigate prevalence of babA2, cagA, iceA1, iceA2, vacA s1/s2 and vacA m1/m2 genotypes in H. pylori from gastric cancer [GC] and gastric ulcer [GU] patients' biopsy samples
Methods: We used FFPE tissues of 35 GC and 10 GU patients' biopsy samples. Using Polymerase Chain Reaction [PCR], detection of H. pylori strain was performed by using specific primers targeting 16S rRNA and ureC encodes for phosphoglucosamine mutase genes. We have identified different virulence genes of H. pylori by PCR
Results: Of all the 45 samples tested, 20 GC and all 10 GU samples were positive for identification of H. pylori using specific genes [16S rRNA and ureC]. The prevalence of babA2 [100%] was significantly higher in GC as compared to GU [40%] samples. The rate of virulence genes vacAs1 was higher in both GU 8 [80%] and GC [100%]
Conclusions: Our study finds that vacAs1am1 and babA2 are most prominent genotypes and may play role in development of Gastric cancer
ABSTRACT
To evaluate the Morbidity, Co-morbidity profile and Disability status among elderly in Civil Hospital Karachi. A Descriptive cross sectional study. The study was conducted at Civil Hospital Karachi from 15 June 2009 to 15 August 2009. Patients aged 60 years and above admitted to selected wards i.e. Medicine, Cardiac medicine, Surgery, Gynecology, E.N.T and Ophthalmology were included except those patients who had psychiatric illnesses, history of trauma and neurological deficits. A questionnaire was designed for assessment of morbidity, co morbidity and disability status of elderly patients. SPSS version 16 was used for data analysis. A total of 220 elderly subjects [60+] were included in the study. Most common morbidities were cerebrovascular accidents [13.6%], chronic liver diseases [7.7%] and hernias [7.7%]. When classified according to organ systems most common disorders were of digestive system [28.6%], nervous system [15%] and malignancies [12.2%]. Hypertension was the most frequent co-morbidity [28.63%], followed by diabetes mellitus [27.2%] and ischemic heart diseases [7.27%]. 63.6% of elderly had minimal disability, 12.3% had moderate disability, 2.7% were severely disabled and 21.4% had no disability. Most of elderly patient admitted to CHK for C.V.A [13.6] Most common disorder were related to Digestive System 28.6%, among Co-Morbidities [Chronic] Hypertension was frequent 28.63%. A large number [78.6%] of elderly patient had some sort of disabilities