ABSTRACT
To determine adipocytes count per unit area in the superficial subcutaneous layer of the abdomen of an adult woman. Cross sectional study. This study was conducted at Department of Anatomy, Isra University Hospital Hyderabad from July 2011 to December 2011. 80 adult women including 40 obese and 40 non obese for different abdominal surgeries visited in the Isra university hospital Hyderabad. Superficial subcutaneous layer of the abdominal adipose tissue were obtained during surgery. Tissues were processed for routine HandE staining. After processing, the number of adipocytes was counted through ocular grid microscopy method. In obese, the mean number of adipocytes was 119.02 +/- 5.81 with range of 100-140 and in non-obese, the mean number of adipocytes was 79.02 +/- 6.02 with range of 50-98. The result showed a significant [p<0.05] increase number of adipocytes in obese women as compare to non obese women. From this study it was conclude that in obese women there is increased number of adipocytes as compare to non obese women. As increased number of adipocytes enhance the cardiovascular as well as metabolic diseases and producing fatal health risks. Therefore there is need to elaborate the cause of disorder and its proper diagnosis through counting or assessment of adipocytes which helpful for management of diseases
ABSTRACT
The present study aims to observe the effect of Nigella Sativa [NS] oil extract on the granulation tissue formation on induced cutaneous wounds in a rabbit model. Experimental study. This study was conducted at the Department of Anatomy, Isra University Hyderabad from May to October, 2012. Twenty male adult Wistar rabbits were studied according to inclusion and exclusion criteria. The rabbits were divided into two groups; Group I. [n=10] skin wounds were treated with 1% pyodine daily and Group II. [n=10] Nigella sativa extract oil was applied on inflicted cutaneous wounds. The animals were sacrificed by over-dose of Ketamine and Xylazil as per protocol and skin wound area was excised. The tissue samples were fixed in marked containers, containing 10% formaldehyde as preservative. The tissue samples were embedded in paraffin, cut into 5 um thick sections and stained with Hematoxylin-Eosin and Masson`s trichrome and examined for histological findings. The data was analyzed on SPSS version 17.0 [Chicago, IL, USA]. The continous variables were analyzed by independent sample t-test. The p-value was taken at