ABSTRACT
The effect of hypercholesterolemia in rabbits was tested on serum protein fractions, lactate dehydrogenase isoenzymes, total protein and glucose levels. The work was extended to estimate certain hepatic enzymes; glucose-6-phosphatase [G-6-Pase], glycogen phosphorylase [GPH], alpha-hydroxybutyrate dehydrogenase [alpha-HBDH], lactate dehydrogenase [LDH] and glycogen content. The corrective action of carnosine and fluvastatin were also evaluated. Twenty five adult male Newzeland rabbits were selected for this study. The animals were divided into five groups. Group 1 served as normal healthy control, group 2; hypercholesterolemic animals [fed on standard rabbit's chow with 1% cholesterol for 12 weeks], group 3; high carnosine dose [50 mg/Kg b. wt.] treated animals, group 4; low carnosine dose [25 mg/Kgb. wt.] treated ones and group 5; fluvastatin [2mg/kg b.wt.] treated group. Treatment started at the last six weeks of cholesterol feeding. Hypercholesterolemic rabbits recorded drastic changes in all parameters under investigation. Carnosine and fluvastatin recorded an enhancement level in major parameters, where high carnosine dose recorded the most potent effect
Subject(s)
Male , Animals, Laboratory , Metabolic Syndrome/drug therapy , Rabbits , Male , Indoles , Treatment OutcomeABSTRACT
The aim of the present study is to investigate the effect of ethanol and the action of carnosine treatment on certain liver parameters, enzymes, total proteins and glycogen in rats. These enzymes including Krebs cycle enzyme: succinate dehydrogenase [SDH]; glycolytic enzymes: lactate dehydrogenase [LDH] d its isoenzymes; glycogenolytic metabolic machineries: glycogen phosphorylase, G-6-Pase and glycoge" drolytic enzymes: acid phosphatase [AP] and alkaline phosphatase [ALP]; amino acid metabolic enzymes aspartate aminotransferase [AST] and alanine aminotransferase [ALT] and nucleic acid catabolic enzyme: 5-nucleotidase. The chronic ethanol-intoxicated rats showed a marked disturbance in all the parameters measured, indicating the toxic effect of ethanol by inducing oxidative stress on liver tissue. Administration of carnosine ameliorated the toxic effects of ethanol
Subject(s)
Male , Animals, Laboratory , Liver/drug effects , Ethanol/toxicity , Alcoholic Intoxication , Protective Agents , Biomarkers , Rats , Liver/enzymology , Glycogen , Chronic DiseaseABSTRACT
The aim of the present study was to investigate the effect of ethanol and the action of carnosine treatment on certain liver parameters; enzymes, total proteins and glycogen in rats. These enzymes including krebs cycle enzyme [succinate dehydrogenase [SDH]], glycolytic enzymes [lactate dehydrogenase [LDH]] and its isoenzymes [glycogenolytic], metabolic machineries [glycogen phosphorylase, G-6-Pase [G-6-P ase]] and glycogen [hydrolytic enzymes] acid phosphatase [AP] and alkaline phosphatase [ALT], amino acid metabolic enzymes [aspartate aminotransferase [AST] and alanine aminotransferase [ALT]] and nucleic acid catabolic enzyme [5'-nucleotidase]. The chronic ethanol-intoxicated rats showed a marked disturbance in all the measured parameters indicating the toxic effect of ethanol by inducing oxidative stress on liver tissue. Administration of carnosine ameliorated the toxic effect of ethanol by enhancement all of the measured parameters