ABSTRACT
Variations in the mineral composition of the medium, its hydrocarbon and nitrogen sources, played a significant role in the productivity of lipids and protein by Candida parapsilosis wild type, KSh 21. Among the petroleum fraction tested were gasolene, kerosene and solar as well as El-Alameen crude oil. Solar at a concentration of 3% v/v proved to be the best carbon source utilized by the organism giving the highest frementation yields. Factors such as fermenation time course, solar level, pH relations [initial and biffered], nature and concentration of nitrogen source, phosphate level and addition of magnesium sulphate and soduim chloride were also tested
Subject(s)
Petroleum , Lipids , ProteinsABSTRACT
Penicillium frequentans, Gibberella fujikuroi and Mortierella isabellina spores were entraped in calcium alginate gel. A 4% glucose and 0.4% ammonium sulfate concentrations were most suitable for a good surface mycelial weft on the beads. The free cells were found to give better growth and higher oxidation of n-tetradecane than the immobilizedd cells of Penicillium frequentans. The utilization of smaller bead size in the entrapment of the same mold greatly activated the formation of oxidation products. A better oxidation was obtained on using an air-lift-reactor