histological study of the pulp responses underneath visible light cured temporary filling material, experimental test

Temporary restorative materials play an important role in protecting the tooth and reserving pulpal health[1]. The reaction of the pulp beneath such a restoration depends on the material ingredients or its sealing properties. This in vivo study was performed to demonstrate the effect of IRM, ZOE, Citadur and Clip temporary restorative materials on the dental pulp and bacterial invasion via the dentinal tubules. Five adult female dogs were used to prepare class V buccal cavities on the posterior teeth. Each segment was filled with a different type of provisional tested filling material. Then the dogs were sacrificed 2 months later. The teeth were grouped, labeled and prepared for histological evaluation. Statistical analysis was performed to compare pulpal responses below various tested restorative materials. Some randomly selected sections from each group were stained with Gram stain for visualization of bacteria. IRM showed the least pulpal reactions, 80% of the cases revealed grade I inflammatory responses. This was followed by ZOE showing only 20% grade I and 70% grade II inflammatory responses. Pulpal response to Citadur varied between grade Il [44%] and grade III [56%] while Clip showed 18% grade II, 46% grade III and 36% grade IV inflammatory reactions. Also, bacterial invasion was demonstrated below Citadur and Clip, which was associated with grades III and IV [unacceptable] that may be due to the observation period of the study

histological study of the oral lesions in erythema multiforme compared with normal oral mucosa

A study of oral lesions in five patients with erythema multiform was carried out to reveal the histopathological and ultrastructural features of this disease in a trial to shed more light on possible diagnostic criteria that may be helpful in the histological diagnosis of this disease, especially if it is limited to the oral cavity, or if it is not accompanied by the characteristic target lesions. The oral lesions were associated with the classical appearance of the skin lesions in three patients, while in the other two cases they were not the only manifestations of the disease. Biopsies of the cheek mucosa were processed for light and electron microscopic examinations. No difference was noticed in the histopathological features of the disease between patients with or without skin involvement. Alterations were observed in whole epithelial layers, basal lamina and the underlying connective tissue

Prostaglandin E2 in cancer of head and neck

Plasma PGE2 was measured in fourteen patients with head and neck carcinoma andin another fourteen healthy subjects. The former group had a significantlyhigher level than the latter. There was a significant correlation betweengrade of the tumor and PGE2 level. Also, bone invasion and PGE2 level inplasma showed a significant correlation. These results were discussed in viewof previous studies and the possible clinical application was highlighted. The biological roles played by prostaglandins [PGs] were illustrated for arelatively short time

Immunohistochemical demonstration of Langerhans cells in irradiated buccal mucosa of patients with head and neck cancer

Biopsy specimens were obtained from irradiated buccal mucosa of patientstreated with Co 60, for head and neck caner. The total dosage, and time-dosefractionation were recorded. Immunohistochemical staining of Langerhans cells[LCs], using anti S-100 protein antiserum, was performed to study the effectof radiotherapy on LCs of the buccal mucosa. Normal non-irradiated buccalmucosa was included in this study, and was served as a control. There was anincrease in the number of LCs after receiving a dose of 1000-2000 rads [r],when compared with normal buccal mucosa, whereas a marked reduction in thenumber of LCs was detected after a dose of 4000-6000 r. Residual LCsfrequently exhibited signs of considerable morphologic alterations