ABSTRACT
OBJECTIVE@#To document the arthropod succession pattern and to identify forensically important species in northeastern Egypt (32° 15' E and 30° 36' N) for the first time.@*METHODS@#Carcasses were exposed in an open area for 60 days during summer season. Ambient daily temperature (maximum and minimum) and relative humidity (RH) were recorded and existing keys were used for identification of different species.@*RESULTS@#During the period of study, the mean of maximum and minimum temperatures were 34.85 °C and 29.2 °C respectively, while the mean of RH was 53.5%. Four stages of decomposition were observed: fresh, bloat, decay and dry. The most abundant orders were found to be Diptera, Coleoptera and Hymenoptera. Arthropods were collected belonging to 4 families of Diptera: Muscidae, Fanniidae, Calliphoridae and Sarcophagidae. While there were 2 families of Coleoptera: Dermestidae and Histeridae. Monomorium species was the only Hymenoptera family in this study.@*CONCLUSION@#The present work provided a basis for further studies dealing with insect colonization of carcasses in different seasons and locations in Egypt.
Subject(s)
Animals , Rabbits , Rats , Arthropods , Classification , Physiology , Coleoptera , Diptera , Egypt , Entomology , Feeding Behavior , Forensic Medicine , Methods , Hymenoptera , Insecta , Classification , Seasons , TemperatureABSTRACT
DNA storage is important to ensure integrity of DNA sample and maintain its availability while investigations. The best known condition for storage of DNA samples is by using Tris-EDT [TE]; as preservative agent, stored at -80[degree sign]C. A potential alternative to TE is trehalose which could stabilize any biological molecule at room temperature [RT]. Assessment of the optimal storage conditions which maintains quality of blood DNA samples with economical advantage. A case-control study using 8 groups of human blood DNA stored at 2 different temperatures [-80 [degree sign]C,RT] and preserved by using TE and trehalose. The effectiveness of storage conditions were tested at certain intervals [at set-up then after 3 and 6 month] using PCR assay of 18s ribosomal gene to evaluate DNA quality. DNA was assessed by running yield gels. PCR success rate were 43.8% and 62.8% using TE and trehalose respectively. After 6 months, PCR success rate were 25% for TE and 62.5% for trehalose [p<0.05]. The relative risk [RR] of poor quality associated with using trehalose is 0.4. Trehalose serves as an alternative to expensive freezer storage. It has a DNA protective effect which helps in preservation even trace DNA while judicial proceedings continue