Comparison of Cytokine Expression in Mesenchymal Stem Cells from Human Placenta, Cord Blood, and Bone Marrow

Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blood (CB) and bone marrow (BM). The cytokine expression profiles of MSCs from BM, CB and placenta (amnion, decidua) were compared by proteome profiler array analysis. The cytokines that were expressed differently, in each type of MSC, were analyzed by real-time PCR. We evaluated 36 cytokines. Most types of MSCs had a common expression pattern including MIF (GIF, DER6), IL-8 (CXCL8), Serpin E1 (PAI-1), GROalpha(CXCL1), and IL-6. MCP-1, however, was expressed in both the MSCs from the BM and the amnion. sICAM-1 was expressed in both the amnion and decidua MSCs. SDF-1 was expressed only in the BM MSCs. Real-time PCR demonstrated the expression of the cytokines in each of the MSCs. The MSCs from bone marrow, placenta (amnion and decidua) and cord blood expressed the cytokines differently. These results suggest that cytokine induction and signal transduction are different in MSCs from different tissues.

Clinical Significance of Preoperative Venogram in Arterio-Venous Shunt Operation

The most common limiting factor in arterio-venous fistula operation (AVF) is the lack of a suitable native vein. So preoperative assessment of venous system is essential for successful results. But simple physical examination alone is not enough to assess in many cases. To evaluate difference between venogram results with physical examination, 20 patients (group A) imaged with venogram preoperatively compared with 20 patients (group B) who examined only physically. In group A, all patients palpated thrill in immediate post operation and one patient revealed fistula occlusion within post operative 2 months. In group B 4 patients revealed failed fistula within 24 hours and 5 fistula failed in 2 months. Twelve patients (>50%) of group A showed different results in venogram compared with physical examination, which influenced type of operation. Physical examination alone was not enough to assess venous system and venogram provided valuable information in AVF constructive surgery.