Sentinel laboratory compliance with best practices in Burkina Faso's antimicrobial resistance surveillance network

Background: Standardising procedures is the best way to harmonise and strengthen the quality of laboratory-based antimicrobial resistance surveillance. Since 2018, Burkina Faso has developed and disseminated the national manual of procedures for performing antibiotic susceptibility tests in sentinel laboratories within its national antimicrobial resistance surveillance network. Objective: Our study aimed to assess these sentinel laboratories' compliance with good practices for antibiotics susceptibility tests. Methods: Four teams evaluated the antimicrobial resistance sentinel sites laboratories throughout Burkina Faso from 19 to 28 September 2022. Eighteen out of 19 sentinel laboratories were evaluated. A four-member technical committee designed and validated the evaluation tool composed of three Microsoft Excel sheets. The evaluation emphasised quality controls for culture media, antibiotic discs and compliance with antimicrobial susceptibility testing procedures by the laboratories. Excel software was used for data recording and graphs and table design. The free R software version 4.2.0 was used for descriptive statistics. An overall score below 80% was considered noncompliance. Results: Most (83.33%) of the sentinel laboratories conducted at least one quality control activity for culture media, and 66.67% conducted at least one quality control activity for antibiotic discs. Over three-quarters (76.47%) of the laboratories were more than 80% compliant with the modified Kirby Bauer antimicrobial susceptibility testing method. Conclusion: The evaluation revealed the noncompliance of sentinel laboratories with the national procedure manual, particularly in the quality control component. What this study adds: This study has provided baseline data on the sentinel laboratories' compliance with the national antimicrobial susceptibility testing procedures manual, particularly in areas performing quality control checks or meeting quality indicators for culture media and antibiotic discs.

Prévalence de la dengue et du paludisme chez les patients fébriles au CHU Souro Sanou de Bobo-Dioulasso

Paludisme et dengue sont des affections à forte morbidité. Les patients présentent souvent les mêmes profils cliniques. Il est difficile, voire impossible de les différencier sans recours à une confirmation biologique. Ceci justifie notre étude dont l'objectif était d'évaluer la prévalence de la dengue et du paludisme chez les patients fébriles consultant au CHUSS de Bobo-Dioulasso au Burkina Faso. Une étude transversale descriptive prospective a été conduite durant le mois d'août 2016 incluant les patients fébriles au CHU-SS de Bobo-Dioulasso. La recherche d'IgM et d'IgG anti Dengue Virus (DENV) a été faite avec le SD BIOLINE IgG/IgM sur 4mL de sang veineux. Le diagnostic de paludisme a été posé par un examen microscopique après coloration au Giemsa d'une goutte épaisse de sang capillaire.Au total 85 patients inclus, 44 hommes, une sex-ratio H/F de 1,07. Les enfants de 0 à 10 ans étaient majoritaires (34,11%). La prévalence des IgM et des IgG anti DENV était de 7,06% et de 22,35% respectivement. Quatre patients étaient porteurs concomitants d'IgM et d'IgG anti DENV. La prévalence du paludisme était de 20%. Pas de co-infection dengue et paludisme. Nos résultats indiquent la circulation de la dengue et du paludisme chez les patients fébriles au CHU de Bobo-Dioulasso. La dengue étant une affection à potentiel épidémique, elle ne doit pas être méconnue chez les patients fébriles

Prévalence de la dengue et du paludisme chez les patients fébriles au CHU Souro Sanou de Bobo-Dioulasso

Paludisme et dengue sont des affections à forte morbidité. Les patients présentent souvent les mêmes profils cliniques. Il est difficile, voire impossible de les différencier sans recours à une confirmation biologique. Ceci justifie notre étude dont l'objectif était d'évaluer la prévalence de la dengue et du paludisme chez les patients fébriles consultant au CHUSS de Bobo-Dioulasso au Burkina Faso. Une étude transversale descriptive prospective a été conduite durant le mois d'août 2016 incluant les patients fébriles au CHU-SS de Bobo-Dioulasso. La recherche d'IgM et d'IgG anti Dengue Virus (DENV) a été faite avec le SD BIOLINE IgG/IgM sur 4mL de sang veineux. Le diagnostic de paludisme a été posé par un examen microscopique après coloration au Giemsa d'une goutte épaisse de sang capillaire. Au total 85 patients inclus, 44 hommes, une sex-ratio H/F de 1,07. Les enfants de 0 à 10 ans étaient majoritaires (34,11%). La prévalence des IgM et des IgG anti DENV était de 7,06% et de 22,35% respectivement. Quatre patients étaient porteurs concomitants d'IgM et d'IgG anti DENV. La prévalence du paludisme était de 20%. Pas de co-infection dengue et paludisme. Nos résultats indiquent la circulation de la dengue et du paludisme chez les patients fébriles au CHU de Bobo-Dioulasso. La dengue étant une affection à potentiel épidémique, elle ne doit pas être méconnue chez les patients fébriles

Digestive fungal flora in asymptomatic subjects in Bobo-Dioulasso, Burkina Faso

<p><b>OBJECTIVE</b>To identify Candida species in asymptomatic subjects in Bobo-Dioulasso (Burkina Faso) by the matrix-assisted laser desorption ionization-time of flight mass spectrometry.</p><p><b>METHODS</b>A cross-sectional study was conducted from January to February 2013 in Bobo-Dioulasso to collect fecal and urine specimens from voluntary donors. Fungal strains were isolated on Sabouraud dextrose agar and analyzed using matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry.</p><p><b>RESULTS</b>A total of 135 samples including stools (78.5%, 106/135) and urine (21.5%, 29/135) were analyzed. The results revealed that fecal specimens contained mainly Candida krusei (C. krusei) (42.5%) followed by Candida albicans (29.3%), Candida glabrata (18.0%) and Candida tropicalis (C. tropicalis) (4.7%). C. krusei (34.6%) was also found to be the most frequently identified in urine samples followed by Candida albicans (27.0%), C. tropicalis (15.4%) and Candida parapsilosis. However, uncommon species such as Candida nivariensis, Candida kefyr, Candida norvegensis, Candida parapsilosis, Candida lusitaniae and Candida robusta were also identified from fecal and urines samples.</p><p><b>CONCLUSIONS</b>This study noted the emergence of species such as C. krusei, Candida glabrata, Candida parapsiolosis, C. tropicalis, Candida nivariensis, Candida norvegensis, and others. It is an imperative to take into account the existence of these species in the therapeutic management of patients in Bobo-Dioulasso.</p>

Digestive fungal flora in asymptomatic subjects in Bobo-Dioulasso, Burkina Faso

Objective: To identify Candida species in asymptomatic subjects in Bobo-Dioulasso (Burkina Faso) by the matrix-assisted laser desorption ionization-time of flight mass spectrometry. Methods: A cross-sectional study was conducted from January to February 2013 in Bobo-Dioulasso to collect fecal and urine specimens from voluntary donors. Fungal strains were isolated on Sabouraud dextrose agar and analyzed using matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry. Results: A total of 135 samples including stools (78.5%, 106/135) and urine (21.5%, 29/135) were analyzed. The results revealed that fecal specimens contained mainly Candida krusei (C. krusei) (42.5%) followed by Candida albicans (29.3%), Candida glabrata (18.0%) and Candida tropicalis (C. tropicalis) (4.7%). C. krusei (34.6%) was also found to be the most frequently identified in urine samples followed by Candida albicans (27.0%), C. tropicalis (15.4%) and Candida parapsilosis. However, uncommon species such as Candida nivariensis, Candida kefyr, Candida norvegensis, Candida parapsilosis, Candida lusitaniae and Candida robusta were also identified from fecal and urines samples. Conclusions: This study noted the emergence of species such as C. krusei, Candida glabrata, Candida parapsiolosis, C. tropicalis, Candida nivariensis, Candida norvegensis, and others. It is an imperative to take into account the existence of these species in the therapeutic management of patients in Bobo-Dioulasso.