ABSTRACT
Objective: To determine the histological effects of Nigella sativa on kidneys against aspirin-induced nephrotoxicity in albino rats. Study Design: An experimental study. Place and Duration of Study: Anatomy Department, University of Health Sciences, Lahore, from January 2014 to December 2015
Methodology: Thirty-two rats were equally divided into four groups A, B, C and D of eight animals each. Group A [control] was provided with single oral dose of 10 mg/100 gm body weight of 1% methyl cellulose. Group B [experimental group] and C [self recovery group] were provided with single oral dose of 1000 mg/kg aspirin. Group D was provided with 250 mg/kg ethanolic extract of Nigella sativa followed by 1000 mg/kg aspirin by oral gavage, after that only extract of Nigella sativa was given for seven days. Animals of groups A, C and D were sacrificed on the 8th day of experiment and that of group B on the second day of experiment. Kidneys were excised and fixed in 10% formalin solution. Hematoxylin, eosin and periodic acid Schiff's reagents were used to stain the kidney tissues. Histological slides were prepared to study proximal convoluted tubules
Results: Histological parameters were normal in control group A. Significant impairment was present in group B. There was no self-recovery in group C. Significant improvement was present in all the parameters of group D
Conclusion: Nigella sativa has protective effect on aspirin-induced nephrotoxicity in rats
ABSTRACT
Objective: To determine the histological effects of Nigella sativa on kidneys against aspirin-induced nephrotoxicity in albino rats
Study Design: An experimental study
Place and Duration of Study: Anatomy Department, University of Health Sciences, Lahore, from January 2014 to December 2015
Methodology: Thirty-two rats were equally divided into four groups A, B, C and D of eight animals each. Group A [control] was provided with single oral dose of 10 mg/100 gm body weight of 1% methyl cellulose. Group B [experimental group] and C [self recovery group] were provided with single oral dose of 1000 mg/kg aspirin. Group D was provided with 250 mg/kg ethanolic extract of Nigella sativa followed by 1000 mg/kg aspirin by oral gavage, after that only extract of Nigella sativa was given for seven days. Animals of groups A, C and D were sacrificed on the 8th day of experiment and that of group B on the second day of experiment. Kidneys were excised and fixed in 10% formalin solution. Hematoxylin, eosin and periodic acid Schiffs reagents were used to stain the kidney tissues. Histological slides were prepared to study proximal convoluted tubules
Results: Histological parameters were normal in control group A. Significant impairment was present in group B. There was no self-recovery in group C. Significant improvement was present in all the parameters of group D
Conclusion: Nigella sativa has protective effect on aspirin-induced nephrotoxicity in rats
ABSTRACT
Objective: To see the effects of Nigella sativa on acetylsalicylic acid-induced nephrotoxicity in albino rats
Study Design: An experimental study
Place and Duration of Study: The Anatomy Department of University of Health Sciences, Lahore, from January 2014 to December 2015
Methodology: Thirty-two female albino rats were divided into four groups. Group A [control] was given single dose of 10 mg/100 gm body weight of 1% methylcellulose, orally. Group B and C were treated with oral 1000 mg/kg acetylsalicylic acid as a single dose. Group D was given 250 mg/kg ethanolic extract of Nigella sativa [NSE] by oral gavage followed by single dose of 1000 mg/kg acetylsalicylic acid on 1st day of experiment; after that only NSE was continued till 7 days. Animals of groups A, C and D were sacrificed on day 8 and that of group B on day 2 of experiment. Cardiac puncture was performed to draw blood from each animal for renal function tests. Animals were evaluated for gross [Paired kidney weight, body weight, relative tissue body weight index] as well as for biochemical [Serum urea and creatinine] parameters
Results: Gross as well as biochemical parameters were markedly impaired in group B, but statistically significant improvement was noticed in Nigella sativa treated group. There was no self recovery in group C
Conclusion: Ethanolic extract of Nigella sativa has a protective role against acetylsalicylic acid-induced nephrotoxicity in albino rats