ABSTRACT
To investigate the mechanism and the suppressive effect of human cytomegalovirus (HCMV) on colony forming unit-megakaryocyte (CFU-MK), semi-solid culture system was used to observe the effect of HCMV AD169 strain on CFU-MK's growth of 18 cord blood samples. HCMV DNA and immediate early (IE) protein mRNA in CFU-MK was detected by PCR and reverse transcription-polymerase chain reaction (RT-PCR). Our results showed that HCMV AD169 significantly suppressed the formation of CFU-MK in vitro. Compared with the mock group, the CFU-MK colonies decreased by 21.6%, 33.8% and 46.3%, respectively, in all the 3 infected groups (P<0.05), suggesting the suppression and the titer of the virus was dose-dependent. Both HCMV DNA and the expression of HCMV IE protein mRNA were positively detected in the colony cells of viral infected group. It is concluded that HCMV AD169 strain could inhibit the differentiation and proliferation of CFU-MK by directly infecting their progenitors. There was early transcription of HCMV IE protein in CFU-MK infected by virus.
Subject(s)
Humans , Antigens, CD34 , Metabolism , Antigens, Viral , Genetics , Cell Division , Cells, Cultured , Cytomegalovirus , Genetics , Physiology , Cytomegalovirus Infections , DNA, Viral , Genetics , Immediate-Early Proteins , Genetics , Megakaryocytes , Cell Biology , Virology , Molecular Sequence Data , Stem Cells , Cell Biology , VirologyABSTRACT
Objective To study the relationship between tissue factor expression and cell metastatic ability in two breast carcinoma cell lines. Methods RT-PCR and flow cytometry(FCM) were used to detect the mRNA and protein expressions of tissue factor(TF) in the breast carcinoma cell lines MCF-7 and MDA-MB231, respectively. The metastatic ability of the two breast carcinoma cell lines was determined using modified Boyden chambers. The data were analyzed by t test. Results The mRNA and protein expression levels of TF in MDA-MB231 were obviously higher than those in MCF-7, and the metastatic ability of MDA-MB231 was stronger than that of MCF-7,which was significantly different between the two cell lines. [WTHZ]Conclusion TF expression was positively related with breast carcinoma metastatic ability, and TF may be a therapeutic target for breast carcinoma.