ABSTRACT
This study aimed to determine the effects of the application of sealant in dental element and its possible influence on the reproductive development and hormonal profile in male Wistar rats as experimental models. Thirty-two male Wistar rats were randomly allocated in four groups, from 23PND (Post Natal Day) to 53PND. They were treated with autopolymerizable or photopolymerizable dental sealant (BISGMA), estradiol cipionate (positive control) or saline solution (negative control). The effects were monitored by the body weight gain, serum concentration of testosterone and estradiol, and by the structure of the seminiferous tubules. Both dental sealants caused alterations in germinative epithelium of the seminiferous tubules, but they didn´t interfere in hormonal serum levels of testosterone or estradiol, neither in body development. It´s possible to conclude that the application of dental sealant in prepubertal period causes damages to the testicular structure, with reduction of the tubular epithelium.
O presente estudo teve como objetivos determinar os efeitos da aplicação de selante de cicatrículas e fissuras em elemento dentário sobre o desenvolvimento reprodutivo masculino pré-púbere e perfil hormonal. Utilizaram-se 32 ratos Wistar machos alocados em 4 grupos dos 23º aos 53º dias de idade e tratados com uma das duas formulações comerciais de selantes dentários à base de dimetacrilato (BISGMA), uma utopolimerizável e outro fotopolimerizável, um grupo com cipionato de estradiol (controle positivo) e outro com solução salina (controle negativo). As possíveis alterações foram avaliadas pelo ganho de peso corporal, concentração sérica de testosterona e estradiol e características estruturais dos testículos. Notou-se que a administração do selante nas suas duas formas, auto-polimerizável e foto-polimerizável, causou alteração no epitélio germinativo dos túbulos seminíferos, mas sem alterações nas concentrações hormonais de estradiol e testosterona. Com base nesse estudo, foi possível concluir que a administração de selantes dentários em ratos Wistar machos pré-púberes, acarreta danos ao epitélio dos túbulos seminíferos constatadas por mudanças na estrutura testicular com redução do epitélio tubular.
Subject(s)
Animals , Male , Spermatogenesis , Puberty , Resins, Synthetic/analysis , Spermatogenesis , Testis/abnormalitiesABSTRACT
This study determined the prevalence of metallo-â-lactamase (MBL)-producing Pseudomonas aeruginosa in two hospitals located in the Southern part of Brazil and compare the performance of two different phenotypic tests. Thirty-one non-repetitive Pseudomonas aeruginosa isolates from various clinical samples from patients admitted to two hospitals located in Rio Grande do Sul, Brazil (twenty-three from a hospital in Porto Alegre City and eight isolates from a hospital in Vale dos Sinos Region). All strains suggestive of possessing MBLs by phenotypic methods were included in this study. Phenotypic detection of MBLs was carried out simultaneously by using both the MBL Etest® and disk approximation test using 2-mercaptopropionic acid close to a ceftazidime disk. Strains positive were further confirmed using molecular techniques for blaVIM, blaIMP and blaSPM-1. The prevalence of MBLs from samplesof inpatients from the hospital located in Porto Alegre was 30.4 percent and that of inpatients from Vale dos Sinos hospital was only 3.1 percent. Only MBL type SPM-1 was detected in these samples by molecular analysis and all were detected by the Etest® MBL strips. The prevalence of P. aeruginosa that produce MBLs can be markedly different in distinct geographical areas, even among different hospitals in the same area. In our study, the EDTA-based method was the only method able to detect all strains harboring the SPM-1 enzyme.