Phytochemical analysis, antibacterial activity of marrubium vulgare L against staphylococcus aureus in vitro

Herbal medicines are the major remedy in traditional medical systems and made a great contribution in maintaining human health and in preventing many infectious diseases. The present study was carried out to determine the potential antibacterial effect of ethanol extracts and essential oil of Marrubium vulgare L. against Staphylococcus aureus which is antibiotic resistant. All 17 strains of S. aureus isolated from nose and throat sample from 160 healthy subjects, hospital staffs and inpatient in the city of Zabol [Amir Al-Momenin hospital, Zabol, south-eastern Iran] were screened during years 2010-2011. In this study, the essential oil of Marrubium vulgare L. obtained by hydrodistillation was analyzed by gas chromatography coupled to mass spectrometry [GC-MS] in order to determine their chemical composition. The minimum inhibitory concentrations were investigated to characterize the antimicrobial activities of this essential oil and extract. Thirty-one components in the oil of Marrubium vulgare were identified. The results demonstrated that the major components of the essential oil were gamma-Eudesmol [11%], Germacrene [10%], D-Citronelly formate [10%], beta-Citronellol [8%], Geranyl tiglate [7.1%], Geranyl formate [6.02%]. The least MIC value of extract M. vulgare was 2.5 mg/mL and the highest MIC value of essential oil M. vulgare was 2.5 mg/mL. This investigation showed that the M. vulgare essential oil and extract has a potent antimicrobial activity against S. aureus. The present studies confirm the use of this essential oil and extract as antibacterial agent. Further research is required to evaluate the practical values of therapeutic applications

effects of capsicum annuum L. extract on the control of single and dual biofilms of common pathogenic strains causing urinary tract infection

Biofilms directly influence the virulence and pathogenicity of a pathogen, it is optimal to employ a strategy that effectively inhibits the formation of biofilm. In this study, the antibacterial and anti-biofilm activities of extract Capsicum annuum L were examined. The eight strains were isolated from urine culture of hospitalized patients; growth and biofilm formation of strains were determined by microtiterplate method. The results revealed that the concentrations of 5 and 10 mg/mL are the most restrain in the biofilm formation of the isolated plates. Results of this study suggest that the extract of C. annuum L may be useful alone to treat bacterial infections