ABSTRACT
Objective: To find out whether Casein Kinase 2 [CK2] and Survivin co-express and correlate in prostate as well as in breast cancer
Study Design: A cross sectional analytical study
Place and Duration of Study: Study was done at Army Medical College and in collaboration with Armed Forces Institute of Pathology [AFIP]. The duration of this research was two years
Material and Methods: The research was authorized by the Ethical Committee of AFIP. CK2 expression was determined by immunohistochemistry in paraffin embedded tissues from established patients of prostate cancer [n=30] and breast adenocarcinoma [n=30]. Correlation of CK2 with Survivin was evaluated. Data were analyzed through SPSS version 20. For studying the correlation between the two proteins, pearson correlation coefficient was calculated. Data was considered at p-value
Results: An expressively strong and affirmative correlation was found among expression of total CK2 and total Survivin in invasive along with non-invasive cases of both prostate and breast cancers. A significantly strong and positive correlation was found between nuclear CK2 and Survivin expression in non-invasive cases of prostate cancer and invasive as well as non-invasive cases of breast cancer. A significantly strong and positive correlation was found only between cytoplasmic CK2 and Survivin expression in non-invasive cases of prostate cancer
Conclusion: CK2 and Survivin expressions have strong and positive correlation in both prostate and breast cancer particularly in non-invasive stage of the cancer
ABSTRACT
Objective: To study the effect of Magnesium supplementation on lipid profile [TC, HDL, LDL, TG, VLDL] and inflammatory markers [CRP s-VCAM 1] in thiazide administered rats
Study Design: Randomized control trial
Place and Duration of Study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi from Feb 2010 to Feb 2011
Material and Methods: Ninety male Sprague Dawley rats, of age 90 to 120 days, divided into three groups: Control, Hydrochlorothiazide [HCTZ] administered control and Hydrochlorothiazide + Magnesium Oxide [HTCZ + Mg2+], administered experimental groups. Levels of inflammatory markers, Magnesium, lipid profile were measured in blood, after three weeks
Results: Mean values in control group were: Serum TG 104.39 mg/dl,TC151.86 mg/dl, HDL76.91 mg/dl, LDL 52.52 mg/dl, VLDL 19.78mg/dl, CRP 1784.27 mg/L, sVCAM-1 564.33ng/ml and serum Magnesium0.85mmol/l. Mean lipid profile values in HCTZ administered control group were Serum TG 150.04 mg/dl, TC 182.92 mg/dl, HDL 71.77 mg/dl, LDL 76.40 mg/dl,VLDL 32.56 mg/dl, CRP 2923.33 mg/L, sVCAM-1 2003.00 ng/ml and serum Magnesium 0.49 mmol/1. Mean lipid profile values in HCTZ + Magnesium administered experimental group were Serum TG 131.69 mg/dl,TC 157.60 mg/dl, HDL 73.55 mg/dl,LDL 58.2 mg/dl,VLDL 25.05mg/dl, CRP 2149.53 mg/L ,sVCAM-l 1516.60 ng/mland serum Magnesium 0.68mmol/l
Conclusion: Levels of sVCAM-1, CRP, TG, TC, LDL and VLDL are increased in HCTZ administered group, with negative correlation with serum magnesium levels. Levels of these parameters decreased in HTCZ + Mg2+ administered group, showing that magnesium supplementation helped in normalizing derangements [p<0.05]
ABSTRACT
CK2 enzyme is up regulated in several cancers. It has many substrates including survivin which is up regulated in cancers
Objectives: to find out the correlation between expression of CK2alpha and survivin and evaluate it as a prospective prognostic marker in pathogenesis of the breast cancer and to find if positive correlation between CK2 and surviving was associated with advancing disease
Study Design: cross Sectional Analytical type of study
Setting: department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi and Armed Forces Institute of Pathology, Rawalpindi
Duration of study: January 2013- December 2014
Methods: the research protocol was approved by Armed Forces Institute of Pathology Ethical Committee. Paraffin embedded tissue sections of diagnosed breast cancer, obtained from AFIP, were used. Immunohistochemistry was performed to determine nuclear and cytoplasm expression of survivin, and CK2 .Scoring done by three histopathologists, independently
Results: total CK2 expression was high in invasive as compared to noninvasive cases [p =0.209]. Cytoplasm and nuclear localization of CK2 in invasive group was a little higher too [p = 0.092] and [p=0.286] respectively. Total survivin expression was high in invasive as compared to non-invasive cases [p= 0.449]. Cytoplasm and nuclear localization of survivin in invasive group was higher as compared to noninvasive group with no significant different [p=0.472] and [p=0.367] respectively. A positive and strong correlation was found in CK2 and survivin expression and localization in both non-invasive as well as invasive groups
Conclusion: CK2 and survivin correlation in cancers can be used in predicting the cancer phenotype and aggression at early stages