ABSTRACT
Despite the widespread prevalence of canine parvovirus disease [CPV] in Iranian dog population, molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. In this study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25 samples from dogs suspected of infection with general symptoms such as depression and anorexia were collected from dogs presented to the veterinary clinic of Shiraz University, Shiraz, Iran. Viral DNA was extracted from feces. Three specific pairs of primers, P2, Pab, and Pb, were used in a PCR assay for differential diagnosis of the virus type. Pab primer pairs detect the new type-strains, CPV-2a and 2b. The primer pairs P2 and Pb detect CPV types 2 and 2b, respectively. Our results showed that 44 individuals with clinical signs of diarrhea and vomiting were positive for CPV-2. 39 individuals [89%] were positive for CPV-2b and 5 individuals [11%] for CPV-2a. Therefore, the CPV-2b was identified as the predominant virus type. All dogs without symptoms of diarrhea and vomiting were CPV-negative. The relationship of breed, age and sex with PCR results was not significant [P>0.05]. For the first time in the country, the causative agent of CPV-2 was identified, and presence of new antigenic variants, CPV-2a and CPV-2b was confirmed
Subject(s)
Animals , Polymerase Chain Reaction , DNA, Viral , Dogs/virologyABSTRACT
A study was carried out in order to find out the status of Giardia spp. and Sarcocystis spp. in pet dogs and stray cats of Shiraz, Fars Province of Iran. Faecal samples of 147 pet dogs and 112 stray cats of different age groups, breeds, and sexes were tested. The stools were examined with the following techniques: direct faecal smears using normal saline, zinc sulfate flotation and formalin-ether concentration technique. Out of a total of 147 pet dogs examined, only one case (0.68%) of Giardia spp. was observed. A total of 3 (2.04%) pet dogs were found positive for Sarcocystis spp. Specimens from stray cats were also examined, however no Giardia spp. trophozoite or cyst was observed in these specimens.