ABSTRACT
Objective/background: Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t[8;14][q24;q32], which involves the c-myc gene [8q24] and the immunoglobulin heavy-chain [IgH] locus [14q32], accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma [DLBCL]. Long-distance polymerase chain reaction [LD-PCR] assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t[8;14] c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL
Methods: In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene [joining region and c switch region] and the myc gene [Exons 2 and 3]
Results: As much as 1.85% of the samples were positive for the c constant region and Exon 2 of the myc gene
Conclusion: LD-PCR can be used for the detection of t[8;14] c-myc/IgH gene rearrangement in patients with DLBCL
ABSTRACT
Morphine is one of the most potent alkaloid in opium, which has substantial medical uses and needs and it is the first active principle purified from herbal source. Morphine has commonly been used for relief of moderate to severe pain as it acts directly on the central nervous system; nonetheless, its chronic abuse increases tolerance and physical dependence, which is commonly known as opiate addiction. Morphine withdrawal syndrome is physiological and behavioral symptoms that stem from prolonged exposure to morphine. A majority of brain regions are hypofunctional over prolonged abstinence and acute morphine withdrawal. Furthermore, several neural mechanisms are likely to contribute to morphine withdrawal. The present review summarizes the literature pertaining to neural mechanisms underlying morphine withdrawal. Despite the fact that morphine withdrawal is a complex process, it is suggested that neural mechanisms play key roles in morphine withdrawal
ABSTRACT
Parkinson's disease [PD] is a neurodegenerative disorder impairing motor, verbal and other abilities. Visual evoked potential [VEP] assessment is a useful method for analysis of visual system and its function. The present study was designed in order to evaluate whether VEP changes are associated with PD. In the present study, 100 subjects encompassing 40 patients with Idiopathic Parkinson's Disease [Idiopathic PD] and 60 aged-matched controls were selected and assigned into case and control groups, respectively. VEP analysis was conducted in either group and the results were compared. In the present study, 16 patients [40%] showed prolonged P100 latency. P100 latency in the case group was significantly longer than in controls. P100 Amplitude was significantly higher in case group than control. There were no significant association between prolonged VEP and sex and diseases duration, in the participants. Also from our participants who suffer from visual hallucination, P100 latency was significantly longer than in the controls. There was a significant association between prolonged P100 latency and severity of disease in the case group. We suggest that prolonged VEP latencies and amplitude are associated with PD and might be associated with a predisposition for visual hallucinations
ABSTRACT
Antifungal drug resistance and few numbers of available drugs limit therapeutic options against fungal infections. The present study was designed to discover new antifungal drugs. This study was carried out in two separate steps, that is, in silico lead identification and in vitro assaying of antifungal potential. A structural data file of a ternary complex of fusicuccin [legend], C terminus of H[+]-ATPase and 14-3-3 regulatory protein [lo9F.pdb file] was used as a model. Computational screening of a virtual 3D database of drug-like molecules was performed and selected small molecules, resembling the functional part of the ligand performing ligand docking, were tested using ArgusLab [4.0.1]. Two lead compounds, 3-Cyclohexan propionic acid [CXP] and 4-phenyl butyric acid [PBA] were selected according to their ligation scores. Standard Strains of Candida albicans and Saccharomyces cerevisiae were used to measure the antifungal potential of the two identified lead compounds against the fungi using micro-well plate dilution assay. Ligation scores for CXP and PBA were -9.33744 and -10.7259 kcal/mol, respectively, and MIC and MFC of CXP and PBA against the two yeasts were promising. The evidence from the present study suggests that CXP and PBA possess potentially antifungals properties
ABSTRACT
It is believed that paraoxonase-2 gene polymorphism is associated with type 2 diabetes. This study is aimed to investigate the association between paraoxonase-2 gene polymorphism and type 2 diabetes in an Iranian population. This study was performed on 200 individuals including 100 diabetics and 100 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes, and PCR-RFLP was carried out. Palindromic sequence in PON2 gene was recognized by Dde1 restriction endonuclease. In order to visualize restriction products, electrophoresis was carried out using polyacrylamide gel [8%] and ethidium bromide staining. The expected PCR product of 331 bp was obtained. Digestion of this product with DdeI showed four Ser homozygotes, three Cys homozygotes, and five Ser311 Cys heterozygotes. The gene frequency of Cys [C] in diabetic subjects was significantly higher than in healthy subjects. This study suggests that an association exists between Ser311 Cys polymorphism and type 2 diabetes mellitus