ABSTRACT
Indian bullfrog Haplobatrachus tigerinus (Daudin) was exposed to sublethal dose (1/3 of LC50 I.E. 1.166 mg/kg) of fenvalerate technical grade and the effect was studied on the specific activity of acetyl cholinesterase in the different tissues of frog viz., brain, muscle, liver, kidney and testis at different time periods viz., 3,6, 12, 24, 48 and 72 hours. The inhibition of specific activity of acetyl cholinesterase was in the order of kidney > brain > muscle > liver > testis. A significant inhibition was noticed in kidney at 12 hours (-64.33%) and no effect was noticed at 3 hours in testis (+0.67%). The AChE activity was inhibited in first three hours of administration of fenvalerate in all the tissue tested. The inhibition continued upto 6 hours or 2 hours in different tissue but the recovery was started by 24 hours and almost completed by 72 hours.
Subject(s)
Acetylcholinesterase/metabolism , Animals , Anura/metabolism , Brain/drug effects , Cholinesterase Inhibitors/toxicity , Kidney/drug effects , Liver/drug effects , Male , Muscle, Skeletal/drug effects , Nitriles , Pyrethrins/toxicity , Spectrophotometry , Testis/drug effects , Time FactorsABSTRACT
Bioaccumulation studies of fenvalerate were conducted on Indian bullfrog Haplobatrachus tigrinus (Daudin) after exposure to sublethal intraperitoneal dose of technical grade fenvalerate (1/3 LD50 i.e. 116.66 microg/kg body weight) at 3, 6, 12, 24, 48 and 72 hours schedule. The tissues viz., muscle, liver, kidney, testis, brain, and whole body accumulation was analysed for residue estimations. In all the tissues, analysed maximum residue was recovered in the initial stages of exposure (3 and 6 hours). However, in brain the residues remained up to 72 hours. In the whole body, analysis after 3 hours of exposure 78.65% residue was recovered and by the time 72 hours passed only, 9.4% residue was recovered. The decline in residue levels along with the period of exposure indicates the fast acting nature of fenvalerate and metabolites.