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1.
Braz. j. microbiol ; 41(3): 581-587, Oct. 2010. ilus, tab
Article in English | LILACS | ID: lil-549398

ABSTRACT

Antibacterial activity of organic and aqueous extracts of Acacia aroma was evaluated against methicillin-resistant Staphylococcus aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus epidermidis. Inhibition of bacterial growth was determined using agar diffusion and bioautographic methods. Among all assayed organic extracts only ethanolic and ethyl acetate extracts presented highest activities against all tested Staphylococcus strains with minimal inhibitory concentration (MIC) values ranging from 2.5 to 10 mg/ml and from 2.5 to 5 mg/ml respectively. The aqueous extracts show little antibacterial activity against Staphylococcus strains. The bioautography assay demonstrated well-defined growth inhibition zones against S. aureus in correspondence with flavonoids and saponins. A. aroma would be an interesting topic for further study and possibly for an alternative treatment for skin infections.


Subject(s)
Humans , Anti-Bacterial Agents , Acacia/growth & development , Plant Structures/growth & development , Plant Extracts/analysis , Methicillin Resistance , Staphylococcus epidermidis/isolation & purification , Bacterial Growth , Methods , Methods
2.
Rev. argent. microbiol ; 29(3): 147-51, jul.-oct. 1997.
Article in Spanish | LILACS | ID: lil-223416

ABSTRACT

Se examinaron 177 muestras de miel para la detección de Clostridium botulinum; 68 procedentes del comercio, 95 de apiarios envasados en origen, 8 de apiarios rurales para consumo familiar y 6 de establecimientos fraccionadores en las provincias de San Luis y Mendoza. Utilizando el método de dilución-centrifugación, se detectó C. botulinum tipo A en 2 muestras de apiarios rurales de San Luis (1,1 o/o); en una de ellas se logró la recuperación de la cepa y el recuento de esporas fue de 55/g de miel. Si bien el porcentaje de positividad fue inferior al de otros países, se recomienda no suministrar miel a niños menores de 1 año como prevención de botulismo del lactante


Subject(s)
Humans , Infant , Botulism/diagnosis , Clostridium botulinum/isolation & purification , Honey/analysis , Spores, Bacterial/isolation & purification , Botulinum Toxins/isolation & purification
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