Validated chromatographic and spectrophotometric methods for analysis of some amoebicide drugs in their combined pharmaceutical preparation

This work is concerned with development and validation of chromatographic and spectrophotometric methods for analysis of Mebeverine HC1 [MEH], Diloxanide furoate [DF] and Metronidazole [MET] in Dimetrol tablets -spectrophotometric and RP-HPLC methods using UV detection. The developed spectrophotometric methods depend on determination of MEH and DF in the combined dosage form using the successive derivative ratio spectra method which depends on derivatization of the obtained ratio spectra in two steps using methanol as a solvent and measuring MEH at 226.4-232.2 nm [peak to peak] and DF at 260.6-264.8 nm [peak to peak]. While MET concentrations were determined using first derivative [[1]D] at lambda = 327 nm using the same solvent. The chromatographic method depends on HPLC separation on ODS column and elution with a mobile phase consisting water: methanol: triethylamine [25: 75: 0.5, by volume, orthophosphoric acid to pH =4]. Pumping the mobile phase at 0.7 ml min[-1] with UV at 230 nm. Factors affecting the developed methods were studied and optimized, moreover, they have been validated as per ICH guideline and the results demonstrated that the suggested methods are reproducible, reliable and can be applied for routine use with short time of analysis. Statistical analysis of the two developed methods with each other using F and student's-t tests showed no significant difference

Prevalence of acinetobacter baumannii at Minia university hospital

Acinetobacter baumannii [A. baumannii] is a rapidly emerging pathogen in the healthcare settings. It usually causes different nosocomial infections, which are usually resistant to several antibiotics. Rapid identification of A. baumannii among hospital patients and the hospital environment is very important to control its spread. Very limited data are available about the prevalence of A. baumannii in Egyptian hospitals particularly at Minia University Hospital [MUH]. In this study, a total of 264 and 109 pus samples were collected from patients suffering from wound and burn infections, respectively. In addition, a total of 549 environmental samples were collected from the environment of MUH Herellea agar was used for isolation of A. baumannii. Identification of the isolates was carried out using culture characteristics, biochemical, staining properties and detection of bla [oxa-51-like] gene; which is intrinsic to A. baumannii; by polymerase chain reaction. A total of 20 [5.4%] and 32 [5.8%] A. baumannii isolates were identified and confirmed by PCR in pus and environmental samples, respectively. While A. baumannii represented the least commonly isolated organism, Klebsiella species [14.5%] and P. aeruginosa [10.4%] were the most frequently isolated aerobic Gram-negative organisms from pus and environmental samples; respectively. Bla axa-51-like gene expression was examined by PCR directly on 48 pus samples after DNA extraction. A. baumannii was identified in 4 pus samples [8.3%] that were negative for culture on here/lea agar and in 8 pus samples [16.7%] that were positive for culture on here/lea agar. So, PCR is a more sensitive and rapid technique for identification of A. baumannii than microbial culture. In conclusion, although A. baumannii does not represent a current major health hazard at MUH, it could lead to significant problems in the future

Sensitivity and specificity of wet mount, culture and PCR in diagnosing trichomonas vaginalis infection in females attending the gynecology clinic of Minia University Hospital

Background and Objectives: trichomoniasis affects approximately 180 million women worldwide. It can have an atypical or even asymptomatic course. Therefore, to accurately diagnose this disease, microbiological investigation is necessary. The aim of this study was to compare wet mount, culture and polymerase chain reaction [PCR]based approaches to establish which method[s] was [were] more efficient in the laboratory diagnosis of trichomoniasis

Study design: one hundred fifty female patients attending the Gynecology Clinic of Minia University Hospital were examined clinically and by different diagnostic tools for detection of T. vaginalis in vaginal samples. For analysis of sensitivity and specificity of the methods used, the PCR technique was used as a gold standard diagnostic tool

Results: T. vaginalis infection was diagnosed in 11 [7.33%], 19 [12.7%], and 50 [33.3%] patients using wet mount, culture in Diamond's medium and PCR techniques, respectively. Although the wet mount for diagnosing T. vaginalis is specific, its sensitivity was poor [22%]. On the other hand, while the sensitivity and specificity of culture technique were 38% and 100%, those of PCR were 95% and 100%, respectively

Conclusion: comparison of different methods for diagnosis of T. vagina/is showed that at least two techniques, such as culture and PCR, have the potential for better diagnosis of infection. PCR detection of T. vaginalis was highly specific and sensitive, but its availability and cost effectiveness is questionable. However, PCR could provide a better alternative for laboratory diagnosis of trichomoniasis by culture