Decreased CD1a + and CD83 + cells in tonsillar squamous cell carcinoma regardless of HPV status

Abstract Dendritic cells (DCs) are specialized antigen-presenting cells that play a critical role in the immune response against human papillomavirus (HPV) infection, and represent a therapeutic target in cancer. Objective: To identify and quantify DCs in tonsillar squamous cell carcinoma (TSCC) under the influence of HPV infection. Methodology: CD1a and CD83 antibodies were used to identify immature dendritic cells and mature dendritic cells by immunohistochemistry in 33 primary TSCC and 10 normal tonsils (NTs), respectively. For the TSCC samples, the number of DCs per area was evaluated in the intra- and peritumoral compartments. For the NTs, the quantification of DCs was evaluated in the intra- and peritonsillar compartments. HPV detection methods were determined according to the ASCO Clinical Practice Guidelines from the College of American Pathologists Guideline (2018). Results: There were fewer intratumoral CD1a+ DCs in the HPV-positive and HPV-negative TSCC groups than in the NT group (p<0.05). In the peritumoral compartment, there were fewer CD83+ DCs in the HPV-positive and HPV-negative TSCC groups than in the NT group (p<0.001). The quantification of DCs subtypes showed no statistical differences between HPV-positive and HPV-negative TSCC groups (p>0.137). Patients with HPV-positive TSCC had significantly better overall survival rate than those with HPV-negative TSCC (p=0.004). Conclusion: Tumor activity contributes to DC depletion regardless of intralesional HPV positivity. An improved prognosis has been reported in patients with HPV-positive TSCC.

Análise do diagnóstico citopatológico realizado em pacientes atendidos na clínica de estomatologia do curso de odontologia da Universidade Federal Fluminense, Nova Friburgo / RJ / Diagnostic cytopathologicalanalysis performed in patients attended by the stomatology clinic of the Universidade Federal Fluminense, Nova Friburgo / RJ

A citopatologia é uma técnica que propicia diagnósticos rápidos para lesões em mucosas, além de ser bastante acessível do ponto de vista econômico. Na odontologia, pode ser um bom exame auxiliar no diagnóstico de lesões bucais, principalmente lesões infecciosas, bem como na detecção precoce do câncer de boca. Objetivos: estabelecer a técnica de citopatologia no laboratório de Patologia Oral do Instituto de Saúde de Nova Friburgo da UFF, bem como avaliar dados demográficos e discutir os diagnósticos citopatológicos desses pacientes. Métodos: foram selecionados 90 pacientes atendidos na clínica de Estomatologia da UFF-NF com lesões orais que tinham indicação para realização da citopatologia oral. Após obtenção de dados demográficos, a coleta de material foi feita, seguida da realização do esfregaço em duas lâminas de vidro. Resultados: Dos 90 pacientes, 54 (60%) eram mulheres e 36 (40%) homens. A idade dos pacientes variou de 30 a 82 anos, com média de 56,60 anos. Desses pacientes, 23 (26%) declararam-se tabagistas, 27 (30%) declararam-se etilistas e 77 (86%) eram usuários de próteses dentárias. Os esfregaços dos 90 pacientes foram diagnosticados de acordo com as classes de Papanicolaou, sendo a maioria, 84 pacientes (93%), diagnosticada como Classe II. Desses 84 pacientes, 34 (40,4%) apresentaram candidíase. Conclusão: após a realização deste trabalho, além da técnica de citopatologia ser estabelecida, pode-se perceber que os esfregaços inflamatórios e apresentando fungo foram predominantes, o que demonstra a boa capacidade diagnóstica da técnica de citopatologia para lesões infecciosas(AU)

Objectives: to establish the technique of cytopathology in the laboratory of Oral Pathology of the Institute of Health of Nova Friburgo of the UFF, as well as to evaluate demographic data and to discuss the cytopathological diagnoses of these patients. Methods: 90 patients attended at the Stomatology clinic of the UFF-NF with oral lesions that had been indicated for oral cytopathology were selected. After obtaining demographic data, the material was collected, followed by the smearing on two glass slides. Results: Of the 90 patients, 54 (60%) were women and 36 (40%) were men. The patients' ages ranged from 30 to 82 years, with a mean of 56.60 years. Of these patients, 23 (26%) declared themselves smokers, 27 (30%) declared themselves alcoholics and 77 (86%) were users of dental prostheses. The smears of the 90 patients were diagnosed according to the classes of Papanicolau, being the majority, 84 patients (93%), diagnosed as Class II. Of these 84 patients, 34 (40.4%) presented candidiasis. Conclusion: after this work, besides the cytopathology technique was established, it can be noticed that the inflammatory smears and presenting fungus were predominant, which demonstrates the good diagnostic capacity of the cytopathology technique for infectious lesions(AU)