ABSTRACT
Staphylococcus aureus (n=157) isolated from intramammary infections in Argentine dairy areas were evaluated for presence of cap5 and cap8 loci. Isolates carrying cap5 and cap8 were serotyped using specific antisera. Sixty four percent of the isolates were genotyped as cap5 or cap8 and 50% of them expressed CP5 or 8.
Subject(s)
Cattle , In Vitro Techniques , Dairy Products/analysis , Mastitis, Bovine/genetics , Phenotype , Polysaccharides, Bacterial/genetics , Polysaccharides, Bacterial/isolation & purification , Polymerase Chain Reaction/methods , Staphylococcus aureus/genetics , Staphylococcus aureus/isolation & purification , Cattle , Genotype , MethodsABSTRACT
Unidirectional fluxes (influx and efflux) and the net flux of palmitate across the hepatocyte membrane were measured in the intact rat liver employing the multiple indicator dilution technique. At albumin concentrations in the range between 0.1 and 0.5 mM the influx rate was 2.3 times greater than that of the net flux. The rate of efflux was somewhat higher than the net flux, indicating that palmitate undergoes an exchange process across the liver cell membranes with efflux to the extracellular albumin site being significant. At. lower albumin concentrations, hower, the influx/net flux approached unit, implying that transport becomes a rate-limiting factor for metabolism