ABSTRACT
Wild and tissue culture raised regenerants of Artemisia amygdalina, a critically endangered and endemic plant of Kashmir and North West Frontier Provinces of Pakistan were screened for the amount of bioactive principles and in particular antimalarial compound artemesinin. Phytochemical screening of extracts revealed the presence of terpenes, alkaloids, phenolics, tannins [polyphenolics], cardiac glycosides and steroids in wild [aerial, inflorescence] and tissue culture regenerants [in vitro grown plant, callus and green house acclimatized plants]. HPLC of Artemisia amygdalina revealed the presence of artemesinin in petroleum ether extracts of wild aerial part, tissue culture raised plant and green house acclimatized plants. Acetonitrile and water in 70:30 ratios at flow rate of 1ml/min was standardised as mobile phase. Retention time for standard chromatogram was 6.7. Wild inflorescences and callus does not produce artemesinin. This is the first report of phytochemical screening and artemesinin estimation of wild and tissue culture raised regenerants of Artemisia amygdalina
Subject(s)
Biological Factors/chemistry , Cardiac Glycosides/chemistry , Phenols/chemistry , Plant Extracts/chemistry , Steroids/chemistry , Tannins/chemistry , Terpenes/chemistry , Tissue Culture Techniques/methods , Alkaloids/chemistry , AntimalarialsABSTRACT
AIM@#To study the chemical constituents and their bioactivity of Polygonum amplexicaule.@*METHODS@#The isolation of compounds was achieved by chromatographic techniques and structure of the isolates was established by UV, IR, HRESI-MS and NMR including 1D and 2D experiments.@*RESULTS@#Bioassay-guided fractionation of an ethanolic extract of Polygonum amplexicaule led to the isolation of a hitherto unidentified compound, 5, 6-dihydropyranobenzopyrone (1) along with nine previously known compounds (2-10). Compounds 2-10 were identified as amplexicine (2), catechin (3), rutin (4), quercetin-3-O-β-D-galactopyranoside (5), chlorogenic acid (6), galloyl glucose (7), caffeic acid (8), gallic acid (9) and scopletin (10).@*CONCLUSION@#Compound 1 is new. Compounds 1-10 exhibited considerable antioxidant activity in a 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay.
Subject(s)
Antioxidants , Chemistry , Pharmacology , Biphenyl Compounds , Metabolism , Coumarins , Chemistry , Pharmacology , Molecular Structure , Picrates , Metabolism , Plant Extracts , Chemistry , Pharmacology , Polygonum , ChemistryABSTRACT
Kashmiri medicinal plant [Prunella vulgaris] was analyzed for its chemical composition and amount of bioactive constituents. The results showed that the herb contains on an average alkaloid [1120 mg%], saponins [350 mg%], phenolics [55.785 mg%] and tannins [52.25 mg%]. The medicinal plant contained carbohydrates [375 mg%], proteins [441.6 mg%] and lipids [2403.8 mg%]. Role of these bioactive principles are discussed according to their folkloric use in Kashmir valley. Besides the herb is of great importance as far as its other clinical application are concerned. This quantitative estimation can be used for comparative evaluation of bioactive constituents with other populations of Prunella vulgaris present in different parts of the world and can be used for selection of superior quality of this herb to use in pharmaceutical industries