ABSTRACT
BACKGROUND:The resorption and unprediction of fat transplantation directly influence the clinical application. The technology of reducing graft resorption by using various cytokines to promote early revascularization of fat transplantation needs to be improved. But the effect is not stable, as the cytokines losing with the bleeding and exudation thus cannot long-term affect the vascular endothelial cells. OBJECTIVE:To investigate the effect of alkaline recombinant basic fibroblast growth factor-dextran formed sustained-release system on the early revascularization of fat grafts. METHODS:The recombinant basic fibroblast growth factor solution 2 mg/L and basic fibroblast growth factor-dextran sustained-release granules were prepared. The fat tissues were selected from the inguinal region of 12 Sprague-Dawley rats for fat transplantation. The left backs of the rats were treated with subcutaneously injection of fat pearl plus recombinant basic fibroblast growth factor solution (control group), and the right backs of rats were treated with subcutaneously injection of fat pearl plus basic fibroblast growth factor-dextran sustained-release granules (sustained-release system group). At 7 and 14 days after treatment, one rat in each group was sacrificed randomly, then ink perfusion microvascular imaging was used to observe the inside of the graft and the density of early formed coated vessels. At 90 days after transplantation, the rest rats were sacrificed, and the grafts were obtained to measure the volume. RESULTS AND CONCLUSION:At 7 and 14 days after transplantation, the number of vessels in the capsule and grafts of the sustained-release system group was higher than that in the control group (P<0.01). At 90 days after transplantation, the volume of grafts in the sustained-release system groups was bigger than that in the control group (P<0.05). The recombinant basic fibroblast growth factor-dextran delivery system could better promote the early revascularization of fat grafts. The experimental results suggest that recombinant basic fibroblast growth factor-dextran sustained-release system can help to reduce the absorption of fat grafts after surgery.
ABSTRACT
Objective:To study the technical operation of self-developed investment (FUS-invest) for titanium crown and bridge. Methods:Orthogonal design was done. Four factors were taken into account, i.e. water/powder ratio, adhesive/hardener ratio, fine powder proportion in refractory and agitation time. And each factor was ranked into three levels. Slurry fluidity, initial setting time and setting time of every experiment group were studied. Grain size graduation of the best group was measured.Results:Fluidity, initial setting time and setting time were most greatly affected by water/powder ratio and secondly by fine powder proportion in refractory. When water/powder ratio was 7.5∶1 and the content of fine powder was 35%, slurry fluidity was better and initial setting time and setting time were appropriate. When the content of fine powder of AFS fineness number 300 was 31.31%, fineness number 100 was (40.09%), fineness number 70 was 18.05% and adhesive/hardness ratio was 200∶1, the perfect titanium castings could be fabricated. Conclution:The best rules of technical operation for perfect titanium castings are water/powder ratio (7.5)∶1, the content of fine powder 35% and adhesive/hardener ratio 200∶1.
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Objective: To obtaine the parameters of tooth mobil ity in people with normal health teeth . Methods:Tooth mob ility of 1 4, 1 4 in 12 subjects (7 males and 5 females, aged 21 to 23 years old) was measured by FX-1 tooth mobility measurement system 6 times a day for 5 days.The data were statisticaly analyzed. Results: The average tooth mobility parameters Q and R varied significantl y (P0.05).Conclusion: The tooth mo bility parameters of S,K,t1 and t2 can steadily reflect the changing of tooth mobility.
ABSTRACT
Objective: To obtain the parameters related to the width of periodontal membrane from tooth mobility signal. Methods: Signals elicited from a tooth model of human maxillary central incisor, periodontal membrane made of silicone rubber and alveolar bone made of resin,were processed utilizing FX-1 tooth mobility measurement system. Results: R value of wave range,slope K and time parameter t2 in the tooth mobility signals were reduced with the increase of the width of periodontal membrane; time parameter t1 was increased with the increase of the width of periodotal membrane;R value and time parameter t2 were negatively correlated. Conclusion: R value and t2 may be the paticular indications reflecting the changing of width of periodontal membrane.